The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station

Cosmic dust samples from the surface of the illuminator of the International Space Station (ISS) were collected by a crew member during his spacewalk. The sampler with tampon in a vacuum container was delivered to the Earth. Washouts from the tampon’s material and the tampon itself were analyzed for...

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Bibliographic Details
Published in:TheScientificWorld 2018, Vol.2018 (2018), p.1-7
Main Authors: Zlatskiy, Igor A., Chichaeva, M. A., Tsygankov, O. S., Morozova, Mariya A., Latyshev, Oleg, Kulikova, N. Y., Kostina, L. V., Eliseeva, Olesja, Shubralova, E. V., Syroeshkin, Anton V., Grebennikova, T. V., Yuzhakov, A. G.
Format: Article
Language:English
Subjects:
Astrobiology
Bacteria
Base Sequence
Circuits
Coastal zone
Cosmic dust
Cosmic Dust - analysis
Deoxyribonucleic acid
DNA
DNA sequencing
DNA, Bacterial - genetics
Dust
Earth (Planet)
Experiments
Genes, Bacterial
Hypotheses
International Space Station
Ionosphere
Laboratories
Mars
Measurement techniques
Nucleotide sequence
Oceans and Seas
Outdoor air quality
Phylogeny
Primers
Researchers
Ribonucleic acid
RNA
RNA, Ribosomal, 16S - genetics
rRNA 16S
Spacecraft
Stratosphere
Vacuum
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Summary:Cosmic dust samples from the surface of the illuminator of the International Space Station (ISS) were collected by a crew member during his spacewalk. The sampler with tampon in a vacuum container was delivered to the Earth. Washouts from the tampon’s material and the tampon itself were analyzed for the presence of bacterial DNA by the method of nested PCR with primers specific to DNA of the genus Mycobacteria, DNA of the strains of capsular bacteria Bacillus, and DNA encoding 16S ribosomal RNA. The results of amplification followed by sequencing and phylogenetic analysis indicated the presence of the bacteria of the genus Mycobacteria and the extreme bacterium of the genus Delftia in the samples of cosmic dust. It was shown that the DNA sequence of one of the bacteria of the genus Mycobacteria was genetically similar to that previously observed in superficial micro layer at the Barents and Kara seas’ coastal zones. The presence of the wild land and marine bacteria DNA on the ISS suggests their possible transfer from the stratosphere into the ionosphere with the ascending branch of the global electric circuit. Alternatively, the wild land and marine bacteria as well as the ISS bacteria may all have an ultimate space origin.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2018/7360147