Biological and Inflammatory Effects of Antigen 5 from Polybia paulista (Hymenoptera, Vespidae) Venom in Mouse Intraperitoneal Macrophages

• Published in: Toxins 2021-11, Vol.13 (12), p.850
• Main Authors: Bazon, Murilo Luiz, Fernandes, Luis Gustavo Romani, Assugeni, Isabela Oliveira Sandrini, Pinto, Lucas Machado, Simioni, Patrícia Ucelli, Zollner, Ricardo de Lima, Braga, Márcia Regina Brochetto
• Format: Article
• Language: English
• Subjects: Allergens; Allergies; Animals; antigen 5; Antigens; Antigens - toxicity; Biological effects; CD80 antigen; CD86 antigen; Cell viability; Cytokines; Flow cytometry; Gene Expression Regulation - drug effects; Hymenoptera; IL-1β; Immune response; Immunomodulation; In vivo methods and tests; Inflammation; inflammatory effects; Lipopolysaccharides; Macrophages; Macrophages, Peritoneal - drug effects; Mice; Mice, Inbred BALB C; Modulation; Molecular weight; Monocyte chemoattractant protein 1; NF-κB protein; Nitric Oxide; Peritoneum; Phenotypes; Phosphorylation; Polybia paulista; Proteins; Transcription Factor RelA - genetics; Transcription Factor RelA - metabolism; Tumor necrosis factor-α; Venom; Vespidae; Wasp Venoms - chemistry; Wasp Venoms - toxicity; Wasps - physiology
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins13120850

The social wasp (Hymenoptera, Vespidae) is highly aggressive, being responsible for many medical occurrences. One of the most allergenic components of this venom is Antigen 5 (Poly p 5). The possible modulation of the in vitro immune response induced by antigen 5 from venom, expressed recombinantly (rPoly p 5), on BALB/c mice peritoneal macrophages, activated or not with LPS, was assessed. Here, we analyzed cell viability changes, expression of the phosphorylated form of p65 NF-κB subunit, nitric oxide (NO), proinflammatory cytokines production, and co-stimulatory molecules (CD80, CD86). The results suggest that rPoly p 5 does not affect NO production nor the expression of co-stimulatory molecules in mouse peritoneal macrophages. On the other hand, rPoly p 5 induced an increase in IL-1β production in non-activated macrophages and a reduction in the production of TNF-α and MCP-1 cytokines in activated macrophages. rPoly p 5 decreased the in vitro production of the phosphorylated p65 NF-κB subunit in non-activated macrophages. These findings suggest an essential role of this allergen in the polarization of functional M2 macrophage phenotypes, when analyzed in previously activated macrophages. Further investigations, mainly in in vivo studies, should be conducted to elucidate Ag5 biological role in the macrophage functional profile modulation.