Transport of Apolipoprotein B-Containing Lipoproteins through Endothelial Cells Is Associated with Apolipoprotein E-Carrying HDL-Like Particle Formation

• Published in: International journal of molecular sciences 2018-11, Vol.19 (11), p.3593
• Main Authors: Yang, Hong, Zhang, Ningya, Okoro, Emmanuel U, Guo, Zhongmao
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Animals; Aorta; Apolipoprotein B; apolipoprotein B-containing lipoprotein; Apolipoprotein E; Apolipoproteins; Apolipoproteins B - metabolism; Apolipoproteins E - metabolism; Arteries; Arteriosclerosis; Atherosclerosis; Caveolae; Cells, Cultured; Cholesterol; Endothelial cells; Endothelial Cells - metabolism; Endothelium; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; High density lipoprotein; high-density lipoprotein formation; Lipoproteins; Lipoproteins, HDL - metabolism; Lipoproteins, LDL - metabolism; Mice; Monolayers; mouse aortic endothelial cells; Plasma; Transcytosis; transendothelial transport; Triglycerides
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19113593

Passage of apolipoprotein B-containing lipoproteins (apoB-LPs), i.e., triglyceride-rich lipoproteins (TRLs), intermediate-density lipoproteins (IDLs), and low-density lipoproteins (LDLs), through the endothelial monolayer occurs in normal and atherosclerotic arteries. Among these lipoproteins, TRLs and IDLs are apoE-rich apoB-LPs (E/B-LPs). Recycling of TRL-associated apoE has been shown to form apoE-carrying high-density lipoprotein (HDL)-like (HDL ) particles in many types of cells. The current report studied the formation of HDL particles by transcytosis of apoB-LPs through mouse aortic endothelial cells (MAECs). Our data indicated that passage of radiolabeled apoB-LPs, rich or poor in apoE, through the MAEC monolayer is inhibited by filipin and unlabeled competitor lipoproteins, suggesting that MAECs transport apoB-LPs via a caveolae-mediated pathway. The cholesterol and apoE in the cell-untreated E/B-LPs, TRLs, IDLs, and LDLs distributed primarily in the low-density (LD) fractions ( ≤ 1.063). A substantial portion of the cholesterol and apoE that passed through the MAEC monolayer was allotted into the high-density (HD) ( > 1.063) fractions. In contrast, apoB was detectable only in the LD fractions before or after apoB-LPs were incubated with the MAEC monolayer, suggesting that apoB-LPs pass through the MAEC monolayer in the forms of apoB-containing LD particles and apoE-containing HD particles.