Characterization of human umbilical cord blood-derived mast cells using high-throughput expression profiling and next-generation knowledge discovery platforms

Mast cells (MCs) are tissue-resident innate immune cells that express the high-affinity receptor for immunoglobulin E and are responsible for host defense and an array of diseases related to immune system. We aimed in this study to characterize the pathways and gene signatures of human cord blood-de...

Full description

Saved in:
Bibliographic Details
Published in:Experimental and molecular pathology 2023-08, Vol.132-133, p.104867-104867, Article 104867
Main Authors: Bakhashab, Sherin, Banafea, Ghalya H., Ahmed, Farid, Alsehli, Haneen, AlShaibi, Huda F., Bagatian, Nadia, Subhi, Ohoud, Gauthaman, Kalamegam, Rasool, Mahmood, Schulten, Hans-Juergen, Pushparaj, Peter Natesan
Format: Article
Language:English
Subjects:
CD117
Cord blood
Immunoglobulin E
Innate immunity
Mast cells
Next generation knowledge discovery
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mast cells (MCs) are tissue-resident innate immune cells that express the high-affinity receptor for immunoglobulin E and are responsible for host defense and an array of diseases related to immune system. We aimed in this study to characterize the pathways and gene signatures of human cord blood-derived MCs (hCBMCs) in comparison to cells originating from CD34− progenitors using next-generation knowledge discovery methods. CD34+ cells were isolated from human umbilical cord blood using magnetic activated cell sorting and differentiated into MCs with rhIL-6 and rhSCF supplementation for 6–8 weeks. The purity of hCBMCs was analyzed by flow cytometry exhibiting the surface markers CD117+CD34−CD45−CD23−FcεR1αdim. Total RNA from hCBMCs and CD34− cells were isolated and hybridized using microarray. Differentially expressed genes were analyzed using iPathway Guide and Pre-Ranked Gene Set Enrichment Analysis. Next-generation knowledge discovery platforms revealed MC-specific gene signatures and molecular pathways enriched in hCBMCs and pertain the immunological response repertoire. [Display omitted] •In vitro differentiated hCBMCs possess the unique mast cell genetic signatures.•hCBMCs have a CD117+CD34−CD45−CD23−FcεR1αdim surface profile.•MC cytokine production and mobility pathways are enriched in hCBMCs.•PI3K is overexpressed in hCBMCs which is essential for homing, and degranulation.
ISSN:0014-4800
1096-0945
DOI:10.1016/j.yexmp.2023.104867