Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to locate individual NPC proteins with

Saved in:
Bibliographic Details
Published in:eLife 2015-12, Vol.4, p.e11349-e11349
Main Authors: Pleiner, Tino, Bates, Mark, Trakhanov, Sergei, Lee, Chung-Tien, Schliep, Jan Erik, Chug, Hema, Böhning, Marc, Stark, Holger, Urlaub, Henning, Görlich, Dirk
Format: Article
Language:English
Subjects:
Antibodies
Antigenic determinants
Bacteria
Biochemistry
Cell Biology
Comparative analysis
Crosslinked polymers
Cysteine
Cystine
epitope mapping
Epitope Mapping - methods
Fluorescence
Fluorescence microscopy
Humans
label displacement
Macromolecular Substances - immunology
Macromolecular Substances - isolation & purification
Mass spectrometry
Nanobodies
nanobody
native purification
nuclear pore complex
Nuclear Pore Complex Proteins - immunology
Nuclear Pore Complex Proteins - isolation & purification
Optical Imaging - methods
Protein binding
Proteins
Sensors
Single-Domain Antibodies - metabolism
site-specific
Staining and Labeling - methods
Tools and Resources
Viral antibodies
Wildlife conservation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to locate individual NPC proteins with
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.11349