Exogenous Salicylic Acid Alleviates NO2 Damage by Maintaining Cell Stability and Physiological Metabolism in Bougainvillea × buttiana ‘Miss Manila’ Seedlings

• Published in: Plants (Basel) 2023-09, Vol.12 (18), p.3283
• Main Authors: Liang, Yuxiang, Li, Dalu, Sheng, Qianqian, Zhu, Zunling
• Format: Article
• Language: English
• Subjects: Air pollution; Antioxidants; Bougainvillea; Bougainvillea × buttiana ‘Miss Manila’; Catalase; cell morphology stability; Chloroplasts; Cytology; Damage; Emissions; Enzymatic activity; Enzyme activity; Enzymes; Function analysis; Heavy metals; Leaves; Metabolism; Metabolites; Morphology; Nitrate reductase; Nitrogen dioxide; Nitrogen metabolism; NO2 stress; Organelles; Outdoor air quality; Photosynthesis; Photosynthetic pigments; physiological metabolisms; Physiology; Pigments; Plant growth; Plant resistance; Pollutants; principal component analysis; Principal components analysis; Reductases; Salicylic acid; Seedlings; Structural stability; Superoxide dismutase
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12183283

Exogenous substances can alleviate plant damage under adverse conditions. In order to explore whether different concentrations of salicylic acid (SA) can play a role in the resistance of Bougainvillea × buttiana ‘Miss Manila’ to nitrogen dioxide (NO2) stress and the relevant mechanisms of their effects, different concentrations of SA were applied locally under the control experiment condition of 4.0 μL·L−1 NO2, and the role of SA in alleviating injury was studied. The findings noted a significant increase in metabolic adaptations and antioxidant enzyme activities following 0.25–0.75 mM SA application (p < 0.05), except 1 mM. Superoxide dismutase (SOD) and catalase (CAT) in particular increased by 21.88% and 59.71%, respectively. Such an increase led to effective control of the reduction in photosynthetic pigments and the photosynthetic rate and protection of the structural stability of chloroplasts and other organelles. In addition, the activity of nitrate reductase (NR) increased by 83.85%, and the content of nitrate nitrogen (NO3−-N) decreased by 29.23% in nitrogen metabolism. Concurrently, a principal component analysis (PCA) and a membership function analysis further indicated that 0.75 mM SA provided the most notable improvement in NO2 resistance among the different gradients. These findings suggest that 0.25–0.75 mM SA can relieve the stress at 4 μL·L−1 NO2 injury by effectively improving the antioxidant enzyme activity and nitrogen metabolizing enzyme activity, protecting the photosynthetic system and cell structure, but 1 mM SA had the opposite effect. In the future, the specific reasons for inhibition of SA at high concentrations and the comprehensive effects of the application of other exogenous compounds should be further studied.