KIT is involved in melanocyte proliferation, apoptosis and melanogenesis in the Rex Rabbit

Melanocytes play an extremely important role in the process of skin and coat colors in mammals which is regulated by melanin-related genes. Previous studies have demonstrated that KIT is implicated in the process of determining the color of the coat in Rex rabbits. However, the effect of KIT on the...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2020-06, Vol.8, p.e9402, Article e9402
Main Authors: Hu, Shuaishuai, Chen, Yang, Zhao, Bohao, Yang, Naisu, Chen, Shi, Shen, Jinyu, Bao, Guolian, Wu, Xinsheng
Format: Article
Language:English
Subjects:
Apoptosis
Cell adhesion & migration
Cell Biology
Cholecystokinin
Cloning
Coloration
Enzymes
Flow cytometry
Free radicals
Gene expression
Genes
Kinases
Laboratory animals
Lysis
Mammals
Melanin
Melanocyte
Melanocytes
Melanogenesis
Microphthalmia-associated transcription factor
Molecular Biology
Mutation
Pigmentation
Proliferation
Protein transport
Proteins
Rabbits
RNA
Skin
Sodium hydroxide
Transfection
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Summary:Melanocytes play an extremely important role in the process of skin and coat colors in mammals which is regulated by melanin-related genes. Previous studies have demonstrated that KIT is implicated in the process of determining the color of the coat in Rex rabbits. However, the effect of KIT on the proliferation and apoptosis of melanocytes and melanogenesis has not been clarified. The mRNA and protein expression levels of KIT were quantified in different coat colored rabbits by qRT-PCR and a Wes assay. To identify whether KIT functions by regulating of melanogenesis, KIT overexpression and knockdown was conducted in melanocytes, and KIT mRNA expression and melanin-related genes TYR, MITF, PMEL and DCT were quantified by qRT-PCR. To further confirm whether KIT influences melanogenesis in melanocytes, melanin content was quantified using NaOH lysis after overexpression and knockdown of KIT. Melanocyte proliferation was estimated using a CCK-8 assay at 0, 24, 48 and 72 h after transfection, and the rate of apoptosis of melanocytes was measured by fluorescence-activated cell sorting. KITmRNA and protein expression levels were significantly different in the skin of Rex rabbits with different color coats (  
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.9402