Use of response surface method for maximizing the production of arginine deiminase by Pseudomonas putida

• Published in: Biotechnology reports (Amsterdam, Netherlands) Netherlands), 2016-06, Vol.10 (C), p.29-37
• Main Authors: Patil, Mahesh D., Shinde, Kiran D., Patel, Gopal, Chisti, Yusuf, Banerjee, Uttam Chand
• Format: Article
• Language: English
• Subjects: Arginine deiminase; Enzyme bioreactors; Enzyme production; Fermentation; Pseudomonas putida KT2440; Submerged culture
• ISSN: 2215-017X; 2215-017X
• DOI: 10.1016/j.btre.2016.03.002

•First report on Arginine deiminase production from Pseudomonas putida using RSM.•Optimum conditions for ADI production were established in shake flasks.•ADI production was assessed in a 14L bioreactor under the optimized conditions.•Repressor effect of aeration on ADI production was observed in 14L bioreactor.•Substantial improvement of 4.5-folds in ADI titre was achieved. Statistically designed experiments were used to optimize the production of arginine deiminase (ADI) by Pseudomonas putida KT2440 in batch culture. A Plackett-Burman design involving eleven factors showed that ADI production was most influenced by the initial pH and the initial concentrations of glucose and yeast extract. A central composite experimental design showed that the optimal values of these factors were 8.0, 10g/L and 12.5g/L, respectively. The other components of the optimal culture medium were bacto peptone 7.5g/L, Triton X–100 0.30% (v/v), and arginine 3g/L, for a culture temperature of 25°C. Compared with the basal medium, the ADI activity in the optimized medium had nearly 4.5-fold increase (4.31U/mL). The optimized medium was then used for a further study of ADI production in a 14L stirred tank bioreactor. The agitation speed and the aeration rates were varied to determine suitable values of these variables.