Megasporogenesis and Megagametogenesis in Coelogyne speciosa subsp. fimbriata (J.J.Sm.) Gravendeel (Orchidaceae Juss.)

Understanding the critical stages in ovule development is essential for optimizing in vitro seed propagation, cryopreservation, and indirect somatic plant embryogenesis. We studied the main stages of the megasporogenesis and megagametogenesis of Coelogyne speciosa subsp. fimbriata (J.J.Sm.) Gravende...

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Published in:International Journal of Plant Biology 2023-03, Vol.14 (1), p.190-198
Main Authors: Kolomeitseva, Galina, Koval, Vladimir, Ryabchenko, Andrey, Babosha, Alexander
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Language:English
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embryo sac
female gametophyte
megaspore
ovule
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Koval, Vladimir
Ryabchenko, Andrey
Babosha, Alexander
description Understanding the critical stages in ovule development is essential for optimizing in vitro seed propagation, cryopreservation, and indirect somatic plant embryogenesis. We studied the main stages of the megasporogenesis and megagametogenesis of Coelogyne speciosa subsp. fimbriata (J.J.Sm.) Gravendeel by confocal laser scanning microscopy. The ovule is anatropic, tenuinucellate, bitegmal, and funicular. The embryo sac is monosporic, Polygonum-type, bipolar, and usually 8-nuclear, but in cases of a reduction in the last mitotic division, it is 6-nuclear. Endosperm formation was not detected. The formation of a functional megaspore occurred 65–70 days after pollination (DAP), while the formation of a mature embryo sac and fertilization occurred after 85–100 DAP.
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subjects embryo sac
female gametophyte
megaspore
ovule
title Megasporogenesis and Megagametogenesis in Coelogyne speciosa subsp. fimbriata (J.J.Sm.) Gravendeel (Orchidaceae Juss.)
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