Endogenous controls and microRNA profile in female patients with obstructive sleep apnea

Recent studies have evaluated the potential of circulating microRNAs (miRNAs) as valuable biomarkers for characterizing obstructive sleep apnea (OSA) in males. The potential use of miRNAs as clinical indicators in females is unknown. The objective is to identify a set of miRNAs to be used as endogen...

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Published in:Scientific reports 2022-02, Vol.12 (1), p.1916-1916, Article 1916
Main Authors: Zapater, Andrea, Benítez, Iván D., Santamaria-Martos, Fernando, Pinilla, Lucía, Targa, Adriano, De Gonzalo-Calvo, David, Torres, Gerard, Mínguez, Olga, Cortijo, Anunciación, Dalmases, Mireia, Barbé, Ferrán, Sánchez-de-la-Torre, Manuel
Format: Article
Language:English
Subjects:
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Adult
Apnea
Biomarkers
Case-Control Studies
Circulating MicroRNA - blood
Circulating MicroRNA - genetics
Female
Females
Gene Expression Profiling
Humanities and Social Sciences
Humans
Longitudinal Studies
MicroRNAs
MicroRNAs - blood
MicroRNAs - genetics
Middle Aged
miRNA
multidisciplinary
Patients
Polysomnography
Population studies
Science
Science (multidisciplinary)
Sex Factors
Sleep
Sleep apnea
Sleep Apnea, Obstructive - blood
Sleep Apnea, Obstructive - diagnosis
Sleep Apnea, Obstructive - genetics
Sleep disorders
Transcriptome
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Summary:Recent studies have evaluated the potential of circulating microRNAs (miRNAs) as valuable biomarkers for characterizing obstructive sleep apnea (OSA) in males. The potential use of miRNAs as clinical indicators in females is unknown. The objective is to identify a set of miRNAs to be used as endogenous controls (ECs) in female patients with OSA. Then, to analyze differences in the miRNA expression profile between patients with and without OSA. This observational, longitudinal study included 85 females with suspected OSA who underwent a polysomnography. OSA was defined as an apnea hypopnea index ≥ 15 events/h. The study population was stratified into 50 OSA patients and 38 non-OSA patients. Exploratory expression profiling of 188 miRNAs consistent and reliable in plasma was performed in a discovery cohort of 21 patients by TaqMan-Low-Density-Array (TLDA). The best ECs were identified by mean centre + standard deviation normalization and concordance correlation restricted normalization. Differentially expressed candidate miRNAs were selected for RT-qPCR validation in a validation cohort of 64 patients. Three circulating miRNAs (miR-30a-5p, miR-93-3p and miR-532-5p) were identified as most stable for use as ECs. Twenty-seven miRNA candidates were identified as potential biomarkers for OSA screening (p value 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-05782-y