Antiproliferative Properties of Triterpenoids by ECIS Method-A New Promising Approach in Anticancer Studies?

Electric cell-substrate impedance sensing is an advanced in vitro impedance measuring system which uses alternating current to determine behavior of cells in physiological conditions. In this study, we used the abovementioned method for checking the anticancer activities of betulin and betulinic aci...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-05, Vol.27 (10), p.3150
Main Authors: Hordyjewska, Anna, Prendecka-Wróbel, Monika, Kurach, Łukasz, Horecka, Anna, Olszewska, Anna, Pigoń-Zając, Dominika, Małecka-Massalska, Teresa, Kurzepa, Jacek
Format: Article
Language:English
Subjects:
Acids
anticancer properties
Antimitotic agents
Antineoplastic agents
Antiproliferatives
Antitumor activity
betulin
Betulinic acid
Breast cancer
Breast Neoplasms
Cancer
Cell growth
Cytology
Cytotoxicity
ECIS
Electric cells
Electric Impedance
Electrodes
Epithelial cells
Epithelium
Female
Health aspects
Humans
Impedance
Lung carcinoma
Metabolism
Morphology
Terpenes
Testing
Triterpenes
Triterpenes - chemistry
Triterpenes - pharmacology
Triterpenoids
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Summary:Electric cell-substrate impedance sensing is an advanced in vitro impedance measuring system which uses alternating current to determine behavior of cells in physiological conditions. In this study, we used the abovementioned method for checking the anticancer activities of betulin and betulinic acid, which are some of the most commonly found triterpenes in nature. In our experiment, the threshold concentrations of betulin required to elicit antiproliferative effects, verified by MTT and LDH release methods, were 7.8 µM for breast cancer (T47D), 9.5 µM for lung carcinoma (A549), and 21.3 µM for normal epithelial cells (Vero). The ECIS results revealed the great potential of betulin and betulinic acid's antitumor properties and their maintenance of cytotoxic substances to the breast cancer T47D line. Moreover, both substances showed a negligible toxic effect on healthy epithelial cells (Vero). Our investigation showed that the ECIS method is a proper alternative to the currently used assay for testing in vitro anticancer activity of compounds, and that it should thus be introduced in cellular routine research. It is also a valuable tool for live-monitoring changes in the morphology and physiology of cells, which translates into the accurate development of anticancer therapies.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27103150