ToBRFV Infects the Reproductive Tissues of Tomato Plants but Is Not Transmitted to the Progenies by Pollination

Tomato brown rugose fruit virus (ToBRFV), a newly identified Tobamovirus, has recently emerged as a significant pathogen of tomato plants (Solanum lycopersicum). The virus can evade or overcome the known tobamovirus resistance in tomatoes, i.e., Tm-1, Tm-2, and its allele Tm-22. ToBRFV was identifie...

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Published in:Cells (Basel, Switzerland) Switzerland), 2022-09, Vol.11 (18), p.2864
Main Authors: Avni, Ben, Gelbart, Dana, Sufrin-Ringwald, Tali, Zemach, Hanita, Belausov, Eduard, Kamenetsky-Goldstein, Rina, Lapidot, Moshe
Format: Article
Language:English
Subjects:
Botanical research
Disease resistance
Disease transmission
Diseases and pests
Environmental aspects
FISH
flower
Flowers & plants
Fluorescence in situ hybridization
Fruits
Germination
Ovules
Plant reproduction
Plant reproductive structures
Plant viruses
Plants
Pollen
Pollination
Reproduction
Reproductive organs
seed
Seeds
Solanum lycopersicum
Stigmas (botany)
Tobamovirus
ToBRFV
tomato
Tomatoes
Virus diseases of plants
Viruses
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Summary:Tomato brown rugose fruit virus (ToBRFV), a newly identified Tobamovirus, has recently emerged as a significant pathogen of tomato plants (Solanum lycopersicum). The virus can evade or overcome the known tobamovirus resistance in tomatoes, i.e., Tm-1, Tm-2, and its allele Tm-22. ToBRFV was identified for the first time only a few years ago, and its interactions with the tomato host are still not clear. We investigated ToBRFV’s presence in the reproductive tissues of tomato using fluorescent in situ hybridization (FISH) and RT-PCR. In infected plants, the virus was detected in the leaves, petals, ovary, stamen, style, stigma, and pollen grains but not inside the ovules. Fruits and seeds harvested from infected plants were contaminated with the virus. To test whether the virus is pollen transmitted, clean mother plants were hand pollinated with pollen from ToBRFV-infected plants and grown to fruit. None of the fruits and seeds harvested from the pollinated clean mother plants contained ToBRFV. Pollen germination assays revealed the germination arrest of ToBRFV-infected pollen. We concluded that ToBRFV might infect reproductive organs and pollen grains of tomato but that it is not pollen transmitted.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells11182864