The Association of an SNP in the EXOC4 Gene and Reproductive Traits Suggests Its Use as a Breeding Marker in Pigs

• Published in: Animals (Basel) 2021-02, Vol.11 (2), p.521
• Main Authors: He, Yingting, Zhou, Xiaofeng, Zheng, Rongrong, Jiang, Yao, Yao, Zhixiang, Wang, Xilong, Zhang, Zhe, Zhang, Hao, Li, Jiaqi, Yuan, Xiaolong
• Format: Article
• Language: English
• Subjects: Animal husbandry; Binding; Birth; Body mass index; Breeding; Chromosomes; commercial pig; Corpus luteum; Domestication; Evolution & development; Gene frequency; Gene mapping; Gene polymorphism; Genes; Genetic testing; Genomes; Genotype & phenotype; Haplotypes; Hogs; Kinases; Markers; Molecular modelling; Nucleotides; Offspring; Ovaries; p53 Protein; Penicillin; Polymerase chain reaction; Polymorphism; promoter; Quantitative trait loci; Reproduction; reproductive traits; Restriction fragment length polymorphism; Single-nucleotide polymorphism; Swine; Transcription factors; Weaning; Zinc
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani11020521

In mammals, the exocyst complex component 4 ( ) gene has often been reported to be involved in vesicle transport. The SNP rs81471943 (C/T) is located in the intron of porcine , while six quantitative trait loci (QTL) within 5-10 Mb around are associated with ovary weight, teat number, total offspring born alive, and corpus luteum number. However, the molecular mechanisms between and the reproductive performance of pigs remains to be elucidated. In this study, rs81471943 was genotyped from a total of 994 Duroc sows, and the genotype and allele frequency of SNP rs81471943 (C/T) were statistically analyzed. Then, the associations between SNP rs81471943 and four reproductive traits, including number of piglets born alive (NBA), litter weight at birth (LWB), number of piglets weaned (NW), and litter weight at weaning (LWW), were determined. Sanger sequencing and PCR restriction fragment length polymorphism (PCR-RFLP) were utilized to identify the rs81471943 genotype. We found that the genotype frequency of CC was significantly higher than that of CT and TT, and CC was the most frequent genotype for NBA, LWB, NW, and LWW. Moreover, 5'-deletion and luciferase assays identified a positive transcription regulatory element in the promoter. After exploring the promoter, SNP -1781G/A linked with SNP rs81471943 (C/T) were identified by analysis of the transcription activity of the haplotypes, and SNP -1781 G/A may influence the potential binding of , E26 transformation specific sequence -like 1 transcription factor ( ), and myeloid zinc finger 1 ( ). These findings provide useful information for identifying a molecular marker of -assisted selection in pig breeding.