Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)

Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic cond...

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Bibliographic Details
Published in:Biological research 2018-11, Vol.51 (1), p.42-42, Article 42
Main Authors: Garcia-Gonzales, Rolando, Pico-Mendoza, José, Quiroz, Karla, Carrasco, Basilio, Cáceres, Pablo, Chong-Perez, Borys, Pino, Hugo, Seiltgens, Marjorie, Greck, Eglis, Caligari, Peter D S
Format: Article
Language:English
Subjects:
Alleles
BIOLOGY
Chilean berry
Deoxyribonucleic acid
DNA
DNA, Plant - genetics
Domestication
Ericaceae
Gaultheria
Gaultheria - genetics
Gaultheria pumila
Genetic diversity
Genetic markers
Genetic Variation
Heterozygosity
Microsatellite
Microsatellite Repeats - genetics
Microsatellites
Polymorphism, Genetic
Population
Population genetics
Population structure
Pyrosequencing
Short Report
Software
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Summary:Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.
ISSN:0717-6287
0716-9760
0717-6287
DOI:10.1186/s40659-018-0193-4