Phytochemical analysis and Enzyme Inhibition Assay of Aerva javanica for Ulcer

Background Aerva javanica (Burm. f.) Juss. ex Schult. (Amaranthacea) is traditionally used for the treatment of wound healings, cough, diarrhoea, ulcer and hyperglycaemia. The current study was aimed to fractionate and isolate bioactive compounds and ultimately to evaluate their anti-ulcereogenic po...

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Published in:BMC chemistry 2012-07, Vol.6 (1), p.76-76, Article 76
Main Authors: Khan, Abdul Wajid, Jan, Saleem, Parveen, Shaista, Khan, Rahmat Ali, Saeed, Asma, Tanveer, Abdul Jabbar, Shad, Anwar Ali
Format: Article
Language:English
Subjects:
Aerva
Aerva javanica
Agriculture
Amides
Ammonia
Biological Chemistry
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Cough
Ethyl acetate fraction
Extraction
Fractionation
Inhibition
LSD
Lysergic acid diethylamide
Polarity
Purification
Research Article
Solvent-solvent extraction
Ulcers
Urease activity
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container_start_page 76
container_title BMC chemistry
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creator Khan, Abdul Wajid
Jan, Saleem
Parveen, Shaista
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description Background Aerva javanica (Burm. f.) Juss. ex Schult. (Amaranthacea) is traditionally used for the treatment of wound healings, cough, diarrhoea, ulcer and hyperglycaemia. The current study was aimed to fractionate and isolate bioactive compounds and ultimately to evaluate their anti-ulcereogenic potential. Results In order to achieve these aims, the fractionation, purifications and then biological potential determination of the isolated compounds was carried out. For purification purpose, initially extraction of the plant material was done with aqueous MeOH in the order of increasing polarity by using solvent-solvent extraction method. Phytochemical analysis revealed the presence of three compounds, 3-hydroxy-4 methoxybenzaldehyde ( 1 ), ursolic acid ( 2 ) and ( E )- N -(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide ( 3 ). Inhibition of urease activity of various fractions revealed that ethyl acetate fraction showed significant activity ( P
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Juss. ex Schult. (Amaranthacea) is traditionally used for the treatment of wound healings, cough, diarrhoea, ulcer and hyperglycaemia. The current study was aimed to fractionate and isolate bioactive compounds and ultimately to evaluate their anti-ulcereogenic potential. Results In order to achieve these aims, the fractionation, purifications and then biological potential determination of the isolated compounds was carried out. For purification purpose, initially extraction of the plant material was done with aqueous MeOH in the order of increasing polarity by using solvent-solvent extraction method. Phytochemical analysis revealed the presence of three compounds, 3-hydroxy-4 methoxybenzaldehyde ( 1 ), ursolic acid ( 2 ) and ( E )- N -(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide ( 3 ). Inhibition of urease activity of various fractions revealed that ethyl acetate fraction showed significant activity ( P &lt;0.05 ) as compared to other fractions. ( E )- N -(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide ( 3 ) showed marked anti ulcer activity ( P &lt;0.05 ). Conclusion These results suggested the mild potential of A. javanica against ulcer.</description><identifier>ISSN: 1752-153X</identifier><identifier>EISSN: 1752-153X</identifier><identifier>EISSN: 2661-801X</identifier><identifier>DOI: 10.1186/1752-153X-6-76</identifier><identifier>PMID: 22849857</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Aerva ; Aerva javanica ; Agriculture ; Amides ; Ammonia ; Biological Chemistry ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Cough ; Ethyl acetate fraction ; Extraction ; Fractionation ; Inhibition ; LSD ; Lysergic acid diethylamide ; Polarity ; Purification ; Research Article ; Solvent-solvent extraction ; Ulcers ; Urease activity</subject><ispartof>BMC chemistry, 2012-07, Vol.6 (1), p.76-76, Article 76</ispartof><rights>Khan et al.; licensee Chemistry Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>2012 Khan et al.; licensee Chemistry Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright ©2012 Khan et al.; licensee Chemistry Central Ltd. 2012 Khan et al.; licensee Chemistry Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b749t-d6adbda6540631151c194b758f99a15305dc19a9dcfb5266b1b5140cc8b02ecd3</citedby><cites>FETCH-LOGICAL-b749t-d6adbda6540631151c194b758f99a15305dc19a9dcfb5266b1b5140cc8b02ecd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1113204848/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1113204848?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22849857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Abdul Wajid</creatorcontrib><creatorcontrib>Jan, Saleem</creatorcontrib><creatorcontrib>Parveen, Shaista</creatorcontrib><creatorcontrib>Khan, Rahmat Ali</creatorcontrib><creatorcontrib>Saeed, Asma</creatorcontrib><creatorcontrib>Tanveer, Abdul Jabbar</creatorcontrib><creatorcontrib>Shad, Anwar Ali</creatorcontrib><title>Phytochemical analysis and Enzyme Inhibition Assay of Aerva javanica for Ulcer</title><title>BMC chemistry</title><addtitle>Chemistry Central Journal</addtitle><addtitle>Chem Cent J</addtitle><description>Background Aerva javanica (Burm. f.) Juss. ex Schult. (Amaranthacea) is traditionally used for the treatment of wound healings, cough, diarrhoea, ulcer and hyperglycaemia. The current study was aimed to fractionate and isolate bioactive compounds and ultimately to evaluate their anti-ulcereogenic potential. Results In order to achieve these aims, the fractionation, purifications and then biological potential determination of the isolated compounds was carried out. For purification purpose, initially extraction of the plant material was done with aqueous MeOH in the order of increasing polarity by using solvent-solvent extraction method. Phytochemical analysis revealed the presence of three compounds, 3-hydroxy-4 methoxybenzaldehyde ( 1 ), ursolic acid ( 2 ) and ( E )- N -(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide ( 3 ). Inhibition of urease activity of various fractions revealed that ethyl acetate fraction showed significant activity ( P &lt;0.05 ) as compared to other fractions. ( E )- N -(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide ( 3 ) showed marked anti ulcer activity ( P &lt;0.05 ). Conclusion These results suggested the mild potential of A. javanica against ulcer.</description><subject>Aerva</subject><subject>Aerva javanica</subject><subject>Agriculture</subject><subject>Amides</subject><subject>Ammonia</subject><subject>Biological Chemistry</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Cough</subject><subject>Ethyl acetate fraction</subject><subject>Extraction</subject><subject>Fractionation</subject><subject>Inhibition</subject><subject>LSD</subject><subject>Lysergic acid diethylamide</subject><subject>Polarity</subject><subject>Purification</subject><subject>Research Article</subject><subject>Solvent-solvent extraction</subject><subject>Ulcers</subject><subject>Urease activity</subject><issn>1752-153X</issn><issn>1752-153X</issn><issn>2661-801X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNkktv1DAQgCMEog-4ckSRuHBJayd-5YK0qkpZqQIOVOJmjR_Z9SqJi51dKfx6nKasdnkILrY18_nz2J4se4XRBcaCXWJOywLT6mvBCs6eZKf7wNOD9Ul2FuMGISow48-zk7IUpBaUn2YfP6_Hweu17ZyGNoce2jG6mBYmv-6_j53Nl_3aKTc43-eLGGHMfZMvbNhBvoEd9Glf3viQ37XahhfZswbaaF8-zufZ3fvrL1cfittPN8urxW2hOKmHwjAwygCjBLEKY4o1roniVDR1DalgRE2KQG10o2jJmMKKYoK0FgqVVpvqPFvOXuNhI--D6yCM0oOTDwEfVhLC4HRrpYVKWVE2psGCmFopRITWVpmqoVqXVXK9m133W9VZo20_BGiPpMeZ3q3lyu9kRThHmCTBYhYo5_8iOM5o38npb-T0N5JJzpLj7WMRwX_b2jjIzkVt2xZ667cx4YQJgUSN_wNFNZ8G-m8UY8IRQnhC3_yCbvw2pHZ4oKoyvRoRibqYKR18jME2-1tiJKeG_P1erw8fd4__7MAEXM5ATKl-ZcPBuX9W_gBjv-s2</recordid><startdate>20120731</startdate><enddate>20120731</enddate><creator>Khan, Abdul Wajid</creator><creator>Jan, Saleem</creator><creator>Parveen, Shaista</creator><creator>Khan, Rahmat Ali</creator><creator>Saeed, Asma</creator><creator>Tanveer, Abdul Jabbar</creator><creator>Shad, Anwar Ali</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SR</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>K9.</scope><scope>KB.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QR</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120731</creationdate><title>Phytochemical analysis and Enzyme Inhibition Assay of Aerva javanica for Ulcer</title><author>Khan, Abdul Wajid ; 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Juss. ex Schult. (Amaranthacea) is traditionally used for the treatment of wound healings, cough, diarrhoea, ulcer and hyperglycaemia. The current study was aimed to fractionate and isolate bioactive compounds and ultimately to evaluate their anti-ulcereogenic potential. Results In order to achieve these aims, the fractionation, purifications and then biological potential determination of the isolated compounds was carried out. For purification purpose, initially extraction of the plant material was done with aqueous MeOH in the order of increasing polarity by using solvent-solvent extraction method. Phytochemical analysis revealed the presence of three compounds, 3-hydroxy-4 methoxybenzaldehyde ( 1 ), ursolic acid ( 2 ) and ( E )- N -(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide ( 3 ). Inhibition of urease activity of various fractions revealed that ethyl acetate fraction showed significant activity ( P &lt;0.05 ) as compared to other fractions. ( E )- N -(4-hydroxy-3-methoxyphenethyl)-3-(4-hydroxy-3-ethoxyphenyl) acryl amide ( 3 ) showed marked anti ulcer activity ( P &lt;0.05 ). Conclusion These results suggested the mild potential of A. javanica against ulcer.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>22849857</pmid><doi>10.1186/1752-153X-6-76</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
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subjects Aerva
Aerva javanica
Agriculture
Amides
Ammonia
Biological Chemistry
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Cough
Ethyl acetate fraction
Extraction
Fractionation
Inhibition
LSD
Lysergic acid diethylamide
Polarity
Purification
Research Article
Solvent-solvent extraction
Ulcers
Urease activity
title Phytochemical analysis and Enzyme Inhibition Assay of Aerva javanica for Ulcer
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