Structural and Functional Characterization of Legionella pneumophila Effector MavL

• Published in: Biomolecules (Basel, Switzerland) Switzerland), 2021-11, Vol.11 (12), p.1802
• Main Authors: Voth, Kevin, Pasricha, Shivani, Chung, Ivy Yeuk Wah, Wibawa, Rachelia R, Zainudin, Engku Nuraishah Huda E, Hartland, Elizabeth L, Cygler, Miroslaw
• Format: Article
• Language: English
• Subjects: Adenosine Diphosphate Ribose - metabolism; ADP-ribosylation; ADP-ribosyltransferase fold; Antibiotics; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; cellular localization; Cloning; Crystal structure; Crystallography, X-Ray; E coli; HEK293 Cells; HeLa Cells; Humans; Immunoprecipitation; Intracellular; Legionella effector; Legionella pneumophila; Legionella pneumophila - enzymology; Legionella pneumophila - growth & development; Legionnaire's disease; Legionnaires' disease; Models, Molecular; Plasmids; Poly(ADP-ribose) polymerase; Protein Binding; Protein Conformation; Protein Folding; protein-protein interactions; Proteins; Ribose; Ribosylation; Structure-function relationships; THP-1 Cells; Type IV Secretion Systems - chemistry; Type IV Secretion Systems - metabolism; Ubiquitin; Ubiquitin-conjugating enzyme; Ubiquitin-Conjugating Enzymes - metabolism; Ubiquitination
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom11121802

is a Gram-negative intracellular pathogen that causes Legionnaires' disease in elderly or immunocompromised individuals. This bacterium relies on the Dot/Icm (Defective in organelle trafficking/Intracellular multiplication) Type IV Secretion System (T4SS) and a large (>330) set of effector proteins to colonize the host cell. The structural variability of these effectors allows them to disrupt many host processes. Herein, we report the crystal structure of MavL to 2.65 Å resolution. MavL adopts an ADP-ribosyltransferase (ART) fold and contains the distinctive ligand-binding cleft of ART proteins. Indeed, MavL binds ADP-ribose with Kd of 13 µM. Structural overlay of MavL with poly-(ADP-ribose) glycohydrolases (PARGs) revealed a pair of aspartate residues in MavL that align with the catalytic glutamates in PARGs. MavL also aligns with ADP-ribose "reader" proteins (proteins that recognize ADP-ribose). Since no glycohydrolase activity was observed when incubated in the presence of ADP-ribosylated PARP1, MavL may play a role as a signaling protein that binds ADP-ribose. An interaction between MavL and the mammalian ubiquitin-conjugating enzyme UBE2Q1 was revealed by yeast two-hybrid and co-immunoprecipitation experiments. This work provides structural and molecular insights to guide biochemical studies aimed at elucidating the function of MavL. Our findings support the notion that ubiquitination and ADP-ribosylation are global modifications exploited by .