Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells

The intestinal absorption and metabolism characteristics of the potentially beneficial polyphenol rutin were studied by measuring the intracellular accumulation and transport of rutin into Caco-2 cells with the sensitive and reliable analytical method of HPLC-coupled tandem mass spectrometry. Rutin...

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Bibliographic Details
Published in:TheScientificWorld 2013-01, Vol.2013 (2013), p.1-8
Main Authors: Wen, Ai-dong, Li, Yan, Miao, Shan, Shi, Xiaopeng, Song, Jinhui, Zhang, Xiaofang
Format: Article
Language:English
Subjects:
ABC transporters
Absorption
Accumulation
Biological products
Caco-2 Cells
Cell interaction
Cell Membrane - metabolism
Cell research
Enzymes
Experiments
Glycoproteins
Hospitals
Humans
Intestinal Absorption - physiology
Liquid chromatography
Mass spectrometry
Membranes
Metabolic Clearance Rate
Metabolism
Metabolites
Polyphenols
Proteins
Rutin - metabolism
Rutin - pharmacokinetics
Small intestine
Studies
Transportation systems
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Summary:The intestinal absorption and metabolism characteristics of the potentially beneficial polyphenol rutin were studied by measuring the intracellular accumulation and transport of rutin into Caco-2 cells with the sensitive and reliable analytical method of HPLC-coupled tandem mass spectrometry. Rutin and glucuronidated rutin were absorbed differently by the basolateral and apical membranes, and rutin showed differential permeability through the apical and basolateral sides. Approximately 33% of the rutin was metabolized to glucuronidated rutin, and the intracellular concentration of glucuronidated rutin was much lower than that of parent rutin. P-glycoprotein and multidrug-resistant proteins 2 and 3 were involved in the transmembrane transport and intracellular accumulation of rutin by Caco-2 cells. These results suggest that a specific transport system mediates rutin movement across the apical membrane in Caco-2 cells and that metabolic enzymes are important for this process.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2013/382350