Reversible Sterilization of Channel Catfish via Overexpression of Glutamic Acid Decarboxylase Gene

• Published in: Animals (Basel) 2024-06, Vol.14 (13), p.1899
• Main Authors: Ye, Zhi, Elaswad, Ahmed, Su, Baofeng, Alsaqufi, Ahmed, Shang, Mei, Bugg, William S, Qin, Guyu, Drescher, David, Li, Hanbo, Qin, Zhenkui, Odin, Ramjie, Makhubu, Nonkonzo, Abass, Nermeen, Dong, Sheng, Dunham, Rex
• Format: Article
• Language: English
• Subjects: Carp; Catfish; channel catfish; Females; gamma-aminobutyric acid; hormone therapy; Plasmids; transgenic fish; transgenic sterilization
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani14131899

The confinement of transgenic fish is essential to prevent their escape and reproduction in natural ecosystems. Reversible transgenic sterilization is a promising approach to control the reproduction of transgenic fish. Therefore, the present study was conducted to develop a reversibly sterile channel catfish ( ) via the transgenic overexpression of the goldfish ( ) glutamic acid decarboxylase (GAD) gene driven by the common carp ( ) β-actin promoter to disrupt normal gamma-aminobutyric acid (GABA) regulation. Three generations of GAD-transgenic fish were produced. All studied generations showed repressed reproductive performance; however, this was not always statistically significant. In F , 5.4% of the transgenic fish showed a sexual maturity score ≥ 4 (maximum = 5) at five years of age, which was lower ( = 0.07) than that of the control group (16.8%). In the spawning experiments conducted on F transgenic fish at six and nine years of age, 45.5% and 20.0% of fish spawned naturally, representing lower values ( = 0.09 and 0.12, respectively) than the percentages in the sibling control fish of the same age (83.3% and 66.7%, respectively). Four of six pairs of the putative infertile six-year-old fish spawned successfully after luteinizing hormone-releasing hormone analog (LHRHa) therapy. Similar outcomes were noted in the three-year-old F fish, with a lower spawning percentage in transgenic fish (20.0%) than in the control (66.7%). In one-year-old F -generation transgenic fish, the observed mean serum gonadotropin-releasing hormone (GnRH) levels were 9.23 ± 2.49 and 8.14 ± 2.21 ng/mL for the females and males, respectively. In the control fish, the mean levels of GnRH were 11.04 ± 4.06 and 9.03 ± 2.36 ng/mL for the females and males, respectively, which did not differ significantly from the control ( = 0.15 and 0.27 for females and males, respectively). There was no significant difference in the estradiol levels of the female transgenic and non-transgenic fish in the one- and four-year-old F -generation fish. The four-year-old F -generation male transgenic fish exhibited significantly ( < 0.05) lower levels of GnRH and testosterone than the control fish. In conclusion, while overexpressing GAD repressed the reproductive abilities of channel catfish, it did not completely sterilize transgenic fish. The sterilization rate might be improved through selection in future generations.