Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response

Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase- luciferase fusion protein that employs bioluminescence resonance energy transfer (BRET) to transduce the...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-11, Vol.23 (23), p.14936
Main Authors: Matveeva, Evgenia G, Stoddard, Andrea K, Zeng, Hui-Hui, Franke, Graham, Bourne, Leslie, Fierke, Carol A, Thompson, Richard B
Format: Article
Language:English
Subjects:
Biology
Bioluminescence
Biosensing Techniques - methods
biosensor
Biosensors
BRET
Carbonic anhydrase
Carbonic Anhydrases - metabolism
Energy transfer
Enzymes
Fluorescence Resonance Energy Transfer - methods
Fusion protein
Ligands
Metal ions
molecular imaging
Organic Chemicals
Proteins
Quantum dots
Selectivity
Sensitivity
Sensors
Trace Elements
Trace metals
Zinc
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Summary:Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase- luciferase fusion protein that employs bioluminescence resonance energy transfer (BRET) to transduce the level of free zinc as a ratio of emission intensities in the blue and orange portions of the spectrum. In addition to high sensitivity (below nanomolar levels) and selectivity, this approach allows both quantitative determination of "free" zinc ion (also termed "mobile" or "labile") using bioluminescence ratios and determination of the presence of the ion above a threshold simply by the change in color of bioluminescence, without an instrument. The carbonic anhydrase metal ion sensing platform offers well-established flexibility in sensitivity, selectivity, and response kinetics. Finally, bioluminescence labeling has proven an effective approach for molecular imaging in vivo since no exciting light is required; the expressible nature of this sensor offers the prospect of imaging zinc fluxes in vivo.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232314936