Highly Sensitive Colorimetric Assay of Cortisol Using Cortisol Antibody and Aptamer Sandwich Assay

In this study, cortisol, which is a key stress hormone, could be detected sensitively via the colorimetric assay of a polycarbonate (PC) and glass substrate by the sandwich assay of cortisol monoclonal antibody (c-Mab) and cortisol specific binding aptamer (c-SBA). A highly sensitive change in color...

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Bibliographic Details
Published in:Biosensors (Basel) 2021-05, Vol.11 (5), p.163
Main Authors: Kim, Yoonjae, Yang, Jongmin, Hur, Hyeyeon, Oh, Seungju, Lee, Hyun Ho
Format: Article
Language:English
Subjects:
antibody aptamer sandwich
Aptamers
Assaying
Biomarkers
Biosensors
c-Mab
c-SBA
colorimetric assay
Colorimetry
Contact angle
Cortisol
Glass substrates
Gold
Hormones
Hydrogen peroxide
Low concentrations
Monoclonal antibodies
Nanoparticles
Polycarbonate
Post traumatic stress disorder
Spectrum analysis
Substrates
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Summary:In this study, cortisol, which is a key stress hormone, could be detected sensitively via the colorimetric assay of a polycarbonate (PC) and glass substrate by the sandwich assay of cortisol monoclonal antibody (c-Mab) and cortisol specific binding aptamer (c-SBA). A highly sensitive change in colorimetry with a limit of detection (LOD) of cortisol of 100 fM could be attained on the optically transparent substrate using the antibody aptamer sandwich (AAS) assay by corresponding stacks of 5 nm gold nanoparticles (Au NPs). The Au NPs were conjugated by the c-SBA and the c-Mab was tethered on the PC and glass substrates. For the AAS method, a simple UV-Vis spectrophotometer was adopted to quantify the cortisol concentrations at an absorbance wavelength of 520 nm. Therefore, this study demonstrates the versatility of the AAS method to measure very low concentrations of cortisol in diagnostic applications.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios11050163