Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium

Thorium is one of the most widespread radioactive elements in natural ecosystems, along with uranium, it is the most important source of nuclear energy. However, the effects of thorium on living organisms have not been thoroughly studied. Marine luminescent bacteria and their enzymes are optimal bio...

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Bibliographic Details
Published in:Bioengineering (Basel) 2021-11, Vol.8 (12), p.194
Main Authors: Kolesnik, Olga V, Rozhko, Tatiana V, Lapina, Maria A, Solovyev, Vladislav S, Sachkova, Anna S, Kudryasheva, Nadezhda S
Format: Article
Language:English
Subjects:
Apoptosis
Bacteria
bioassay
Bioassays
Bioengineering
Bioluminescence
Biophysics
Cell division
Enzymes
Exposure
Hormesis
luminous bacteria
NADH
Nicotinamide adenine dinucleotide
Nuclear energy
Nuclear reactors
Oxidation
Phosphates
Physiology
Potassium
Radiation
Radioisotopes
Reactive oxygen species
Reagents
Thorium
Thorium-232
Toxicity
Uranium
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Summary:Thorium is one of the most widespread radioactive elements in natural ecosystems, along with uranium, it is the most important source of nuclear energy. However, the effects of thorium on living organisms have not been thoroughly studied. Marine luminescent bacteria and their enzymes are optimal bioassays for studying low-dose thorium exposures. Luminescent bioassays provide a quantitative measure of toxicity and are characterized by high rates, sensitivity, and simplicity. It is known that the metabolic activity of bacteria is associated with the production of reactive oxygen species (ROS). We studied the effects of thorium-232 (10 -10 M) on and bacterial enzymatic reactions; kinetics of bacterial bioluminescence and ROS content were investigated in both systems. Bioluminescence activation was revealed under low-dose exposures (
ISSN:2306-5354
2306-5354
DOI:10.3390/bioengineering8120194