Exploring Albumin Functionality Assays: A Pilot Study on Sepsis Evaluation in Intensive Care Medicine

Human serum albumin (HSA) as the most abundant plasma protein carries multifunctional properties. A major determinant of the efficacy of albumin relies on its potent binding capacity for toxins and pharmaceutical agents. Albumin binding is impaired in pathological conditions, affecting its function...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2023-08, Vol.24 (16), p.12551
Main Authors: Klinkmann, Gerd, Waterstradt, Katja, Klammt, Sebastian, Schnurr, Kerstin, Schewe, Jens-Christian, Wasserkort, Reinhold, Mitzner, Steffen
Format: Article
Language:English
Subjects:
Albumin
binding capacity
Binding sites
Blood proteins
Creatinine
critical care
Efficiency
electron paramagnetic resonance
Ethanol
Fatty acids
Infection
Intensive care
intensive care medicine
Liver
Medical prognosis
Medical research
Medicine, Experimental
Mortality
Patients
Proteins
Regression analysis
Sepsis
Septic shock
Spectrum analysis
spin-probe technique
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human serum albumin (HSA) as the most abundant plasma protein carries multifunctional properties. A major determinant of the efficacy of albumin relies on its potent binding capacity for toxins and pharmaceutical agents. Albumin binding is impaired in pathological conditions, affecting its function as a molecular scavenger. Limited knowledge is available on the functional properties of albumin in critically ill patients with sepsis or septic shock. A prospective, non-interventional clinical trial assessed blood samples from 26 intensive care patients. Albumin-binding capacity (ABiC) was determined by quantifying the unbound fraction of the fluorescent marker, dansyl sarcosine. Electron paramagnetic resonance fatty acid spin-probe evaluated albumin’s binding and detoxification efficiencies. Binding efficiency (BE) reflects the strength and amount of bound fatty acids, and detoxification efficiency (DTE) indicates the molecular flexibility of patient albumin. ABiC, BE, and DTE effectively differentiated control patients from those with sepsis or septic shock (AUROC > 0.8). The diagnostic performance of BE showed similarities to procalcitonin. Albumin functionality correlates with parameters for inflammation, hepatic, or renal insufficiency. Albumin-binding function was significantly reduced in critically ill patients with sepsis or septic shock. These findings may help develop patient-specific algorithms for new diagnostic and therapeutic approaches.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241612551