Loading…

TaARPC3, Contributes to Wheat Resistance against the Stripe Rust Fungus

The actin cytoskeleton participates in numerous cellular processes, including less-characterized processes, such as nuclear organization, chromatin remodeling, transcription, and signal transduction. As a key regulator of actin cytoskeletal dynamics, the actin related protein 2/3 complex (Arp2/3 com...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in plant science 2017-07, Vol.8, p.1245-1245
Main Authors: Qi, Tuo, Wang, Juan, Sun, Qixiong, Day, Brad, Guo, Jun, Ma, Qing
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The actin cytoskeleton participates in numerous cellular processes, including less-characterized processes, such as nuclear organization, chromatin remodeling, transcription, and signal transduction. As a key regulator of actin cytoskeletal dynamics, the actin related protein 2/3 complex (Arp2/3 complex) controls multiple developmental processes in a variety of tissues and cell types. To date, the role of the Arp2/3 complex in plant disease resistance signaling is largely unknown. Herein, we identified and characterized wheat ARPC3, , which encodes the C3 subunit of the Arp2/3 complex. Expression of in the mutant of Δ resulted in complementation of stress-induced phenotypes in , as well as restore wild-type cell shape malformations. TaARPC3 was found predominantly to be localized in the nucleus and cytoplasm when expressed transiently in wheat protoplast. was significantly induced in response to avirulent race of f. sp. ( ). Knock-down of by virus-induced gene silencing resulted in a reduction of resistance against through a specific reduction in actin cytoskeletal organization. Interestingly, this reduction was found to coincide with a block in reactive oxygen species (ROS) accumulation, the hypersensitive response (HR), an increase in mRNA accumulation, and the growth of . Taken together, these findings suggest that is a key subunit of the Arp2/3 complex which is required for wheat resistance against , a process that is associated with the regulation of the actin cytoskeleton.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2017.01245