Loading…
TaARPC3, Contributes to Wheat Resistance against the Stripe Rust Fungus
The actin cytoskeleton participates in numerous cellular processes, including less-characterized processes, such as nuclear organization, chromatin remodeling, transcription, and signal transduction. As a key regulator of actin cytoskeletal dynamics, the actin related protein 2/3 complex (Arp2/3 com...
Saved in:
Published in: | Frontiers in plant science 2017-07, Vol.8, p.1245-1245 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | The actin cytoskeleton participates in numerous cellular processes, including less-characterized processes, such as nuclear organization, chromatin remodeling, transcription, and signal transduction. As a key regulator of actin cytoskeletal dynamics, the actin related protein 2/3 complex (Arp2/3 complex) controls multiple developmental processes in a variety of tissues and cell types. To date, the role of the Arp2/3 complex in plant disease resistance signaling is largely unknown. Herein, we identified and characterized wheat ARPC3,
, which encodes the C3 subunit of the Arp2/3 complex. Expression of
in the
mutant of
Δ
resulted in complementation of stress-induced phenotypes in
, as well as restore wild-type cell shape malformations. TaARPC3 was found predominantly to be localized in the nucleus and cytoplasm when expressed transiently in wheat protoplast.
was significantly induced in response to avirulent race of
f. sp.
(
). Knock-down of
by virus-induced gene silencing resulted in a reduction of resistance against
through a specific reduction in actin cytoskeletal organization. Interestingly, this reduction was found to coincide with a block in reactive oxygen species (ROS) accumulation, the hypersensitive response (HR), an increase in
mRNA accumulation, and the growth of
. Taken together, these findings suggest that
is a key subunit of the Arp2/3 complex which is required for wheat resistance against
, a process that is associated with the regulation of the actin cytoskeleton. |
---|---|
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2017.01245 |