Combined Antimicrobial Blue Light and Antibiotics as a Tool for Eradication of Multidrug-Resistant Isolates of Pseudomonas aeruginosa and Staphylococcus aureus: In Vitro and In Vivo Studies

Increased development of resistance to antibiotics among microorganisms promotes the evaluation of alternative approaches. Within this study, we examined the efficacy of antimicrobial blue light (aBL) with routinely used antibiotics against multidrug-resistant isolates of Pseudomonas aeruginosa and...

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Bibliographic Details
Published in:Antioxidants 2022-08, Vol.11 (9), p.1660
Main Authors: Woźniak, Agata, Grinholc, Mariusz
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial agents
Bioluminescence
blue light
Cell envelopes
Chloramphenicol
Ciprofloxacin
Colistin
Drug dosages
Drug resistance
Experiments
Fosfomycin
Fusidic acid
Light emitting diodes
Linezolid
Medical research
mouse model
Multidrug resistance
Pathogens
Permeability
Photoinactivation
porphyrins
Pseudomonas aeruginosa
rose bengal
Staphylococcus aureus
Virulence
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Summary:Increased development of resistance to antibiotics among microorganisms promotes the evaluation of alternative approaches. Within this study, we examined the efficacy of antimicrobial blue light (aBL) with routinely used antibiotics against multidrug-resistant isolates of Pseudomonas aeruginosa and Staphylococcus aureus as combined alternative treatment. In vitro results of this study confirm that both S. aureus and P. aeruginosa can be sensitized to antibiotics, such as chloramphenicol, linezolid, fusidic acid or colistin, fosfomycin and ciprofloxacin, respectively. The assessment of increased ROS production upon aBL exposure and the changes in cell envelopes permeability were also goals that were completed within the current study. Moreover, the in vivo experiment revealed that, indeed, the synergy between aBL and antibiotic (chloramphenicol) occurs, and the results in the reduced bioluminescence signal of the S. aureus Xen31 strain used to infect the animal wounds. To conclude, we are the first to present the possible mechanism explaining the observed synergies among photoinactivation with blue light and antibiotics in the term of Gram-positive and Gram-negative representatives.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox11091660