The long non-coding RNA lncRNA-DRNR enhances infectious bronchitis virus replication by targeting chicken JMJD6 and modulating interferon-stimulated genes expression via the JAK-STAT signalling pathway

• Published in: Veterinary research (Paris) 2024-11, Vol.55 (1), p.141-141, Article 141
• Main Authors: Yan, Wenjun, Fu, Xue, Li, Hao, Wang, Kailu, Song, Cailiang, Hou, Chengyao, Lei, Cangwei, Wang, Hongning, Yang, Xin
• Format: Article
• Language: English
• Subjects: Animals; Avian Proteins - genetics; Avian Proteins - metabolism; Biological response modifiers; Bronchitis; Cell Line; Chickens; chJMJD6; Coronavirus Infections - genetics; Coronavirus Infections - veterinary; Coronavirus Infections - virology; Coronaviruses; disease severity; Ethylenediaminetetraacetic acid; etiological agents; Gene expression; Gene Expression Regulation; Genes; Health aspects; Immune response; infectious bronchitis; Infectious bronchitis virus; Infectious bronchitis virus - genetics; Infectious bronchitis virus - immunology; Infectious bronchitis virus - physiology; interferon; Interferons - genetics; Interferons - metabolism; Janus Kinases - genetics; Janus Kinases - metabolism; Jumonji Domain-Containing Histone Demethylases - genetics; Jumonji Domain-Containing Histone Demethylases - metabolism; Life Sciences; Long non-coding RNA; Medical research; Medicine, Experimental; Methylation; non-coding RNA; pathogenesis; Poultry Diseases - genetics; Poultry Diseases - immunology; Poultry Diseases - virology; Poultry industry; Proteins; RNA; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Signal Transduction; Virus Replication; viruses
• ISSN: 1297-9716; 0928-4249; 1297-9716
• DOI: 10.1186/s13567-024-01396-6

Infectious bronchitis virus (IBV) is the causative agent of infectious bronchitis (IB), a severe disease that primarily affects young chickens and poses a significant challenge to the global poultry industry. Understanding the complex interaction between the virus and its host is vital for developing innovative antiviral strategies. Long non-coding RNA (lncRNA) plays a crucial role in regulating host antiviral immune responses. Our previous studies have shown that IBV infection disrupts the stability of lncRNA in host cells, indicating a potential regulatory role for lncRNA in IBV pathogenesis. It is still not clear how lncRNA precisely modulates IBV replication. In this study, we observed down-regulation ofMSTRG.26120.58 (named lncRNA-DRNR) expression in various chicken cell lines upon IBV infection. We demonstrated that silencing lncRNA-DRNR using siRNA enhances intracellular replication of IBV. Through exploring genes encoding proteins upstream and downstream of lncRNA-DRNR within a 100 kb range, we identified chJMJD6 (chicken JMJD6) as a potential target gene negatively regulated by lncRNA-DRNR expression levels. Furthermore, chJMJD6 inhibits STAT1 methylation, thereby affecting the induction of interferon-stimulated genes (ISGs) through the activation of the IFN-β-mediated JAK-STAT signalling pathway, ultimately promoting the intracellular replication of IBV. In summary, our findings reveal the critical role played by lncRNA-DRNR during IBV infection, providing novel insights into mechanisms underlying coronavirus-induced disruption in lncRNA stability.