Survey of False-positive Reactivity of Latex Agglutination Test for Kala-azar (Katex) without Urine Sample Boiling Process in Autoimmune Patients

Latex agglutination test for Kala-azar (KAtex) is an easy, inexpensive, and field-applicable antigen detection test. However, the main drawback of this method is the boiling step applied to remove false positivity of the test. This study was conducted to survey false positivity results of latex aggl...

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Bibliographic Details
Published in:Iranian journal of public health 2017-06, Vol.46 (6), p.804-810
Main Authors: Ghatee, Mohammad Amin, Kanannejad, Zahra, Sharifi, Iraj, Askari, Asma, Bamorovat, Mehdi
Format: Article
Language:English
Subjects:
Agglutination
Arthritis
Autoimmune diseases
Autoimmune patients
Boiling
Chronic conditions
False positivity
Health risk assessment
Immune system
KAtex
Latex
Latex agglutination
Original
Patients
Pemphigus
Rheumatoid arthritis
Rheumatoid factor
Sampling methods
Scleroderma
Systemic lupus erythematosus
Urine
Vasculitis
Vitiligo
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Summary:Latex agglutination test for Kala-azar (KAtex) is an easy, inexpensive, and field-applicable antigen detection test. However, the main drawback of this method is the boiling step applied to remove false positivity of the test. This study was conducted to survey false positivity results of latex agglutination test for KAtex without boiling process in urine of some autoimmune patients. Ninety-two urine samples from autoimmune patients including systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), scleroderma, autoimmune vasculitis, vitiligo, pemphigus and Wagner cases and 20 urine samples from healthy individuals were collected from Kerman Province in Southeastern Iran in 2010-2011. All urine samples were checked by KAtex after boiling for 5 min false positivity rate of the test was surveyed in different healthy and patients groups while boiling process was removed. Rheumatoid factor (RF) then was checked in sera of all cases to evaluate the relationship between RF and KAtex false positivity. All samples represented negative results with KAtex when boiling was performed (100% specificity). Then, 20% positivity was evident in healthy cases. False-positive reactivity was more prominent observed in patient groups than healthy individuals, except in vitiligo. However, a significant difference was only observed in RA group (
ISSN:2251-6085
2251-6093