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Influence of infected root dentin on the bond strength of a self-adhesive resin cement

Aim: The aim of this study was to determine the bond strength (BS) of a self-adhesive resin cement to the contaminated root dentin. Materials and Methods: The crown and apical third of twenty single-rooted teeth were removed. The root canals were flared and 1-mm-thick root sections were obtained. Th...

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Published in:Contemporary clinical dentistry 2018-01, Vol.9 (1), p.26-30
Main Authors: Delai, Débora, Tedesco, Maybell, de Almeida, Josiane, Chain, Marcelo, da Silveira Teixeira, Cleonice, Felippe, Mara, Felippe, Wilson
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container_title Contemporary clinical dentistry
container_volume 9
creator Delai, Débora
Tedesco, Maybell
de Almeida, Josiane
Chain, Marcelo
da Silveira Teixeira, Cleonice
Felippe, Mara
Felippe, Wilson
description Aim: The aim of this study was to determine the bond strength (BS) of a self-adhesive resin cement to the contaminated root dentin. Materials and Methods: The crown and apical third of twenty single-rooted teeth were removed. The root canals were flared and 1-mm-thick root sections were obtained. The sections were rinsed, dried, and sterilized. The control group (n=20) was composed of one section of each third, which remained immersed in sterile trypticase soy broth (TSB) for 2 months. The other sections comprised the experimental group (n = 40) and were immersed in a suspension of Enterococcus faecalis. The culture medium was changed at every 4 days for 2 months. The sections were rinsed with distilled water, dried, and the root canal space was fi lled with the self-adhesive resin cement RelyX™ U200. After 24 h, the push-out test was performed and the types of interface failure were observed on a stereo microscope. Statistical Analysis: Data were statistically analyzed by the nonparametric Mann-Whitney test (α=5%). Results: A significant reduction was observed in the BS of resin cement to the contaminated dentin compared to the healthy dentin, for both thirds analyzed (P < 0.05). The BS was signifi cantly greater at the cervical third compared to the middle third for specimens in the experimental group (P < 0.05). Adhesive and mixed failures were observed more frequently in specimens contaminated with E. faecalis. Conclusion: Bacterial contamination negatively infl uenced the BS of the self-adhesive resin cement to the root dentin, and there was a predominance of adhesive and mixed failures.
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Materials and Methods: The crown and apical third of twenty single-rooted teeth were removed. The root canals were flared and 1-mm-thick root sections were obtained. The sections were rinsed, dried, and sterilized. The control group (n=20) was composed of one section of each third, which remained immersed in sterile trypticase soy broth (TSB) for 2 months. The other sections comprised the experimental group (n = 40) and were immersed in a suspension of Enterococcus faecalis. The culture medium was changed at every 4 days for 2 months. The sections were rinsed with distilled water, dried, and the root canal space was fi lled with the self-adhesive resin cement RelyX™ U200. After 24 h, the push-out test was performed and the types of interface failure were observed on a stereo microscope. Statistical Analysis: Data were statistically analyzed by the nonparametric Mann-Whitney test (α=5%). Results: A significant reduction was observed in the BS of resin cement to the contaminated dentin compared to the healthy dentin, for both thirds analyzed (P &lt; 0.05). The BS was signifi cantly greater at the cervical third compared to the middle third for specimens in the experimental group (P &lt; 0.05). Adhesive and mixed failures were observed more frequently in specimens contaminated with E. faecalis. Conclusion: Bacterial contamination negatively infl uenced the BS of the self-adhesive resin cement to the root dentin, and there was a predominance of adhesive and mixed failures.</description><identifier>ISSN: 0976-237X</identifier><identifier>EISSN: 0976-2361</identifier><identifier>DOI: 10.4103/ccd.ccd_683_17</identifier><identifier>PMID: 29599579</identifier><language>eng</language><publisher>India: Wolters Kluwer India Pvt. Ltd</publisher><subject>Adhesion ; Adhesive systems ; Adhesives ; Bond strength ; Cement ; Collagen ; contaminated dentin ; Contamination ; Data processing ; Dental cement ; Dental crowns ; Dentin ; Dentistry ; Enamel ; Endodontics ; Failure analysis ; Microorganisms ; Original ; push-out test ; Resins ; Root canal therapy ; Root canals ; Statistical analysis ; Teeth</subject><ispartof>Contemporary clinical dentistry, 2018-01, Vol.9 (1), p.26-30</ispartof><rights>COPYRIGHT 2018 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications &amp; Media Pvt. Ltd. Jan/Mar 2018</rights><rights>Copyright: © 2018 Contemporary Clinical Dentistry 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863404/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2011135776?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,25753,27923,27924,27925,37012,37013,44590,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29599579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Delai, Débora</creatorcontrib><creatorcontrib>Tedesco, Maybell</creatorcontrib><creatorcontrib>de Almeida, Josiane</creatorcontrib><creatorcontrib>Chain, Marcelo</creatorcontrib><creatorcontrib>da Silveira Teixeira, Cleonice</creatorcontrib><creatorcontrib>Felippe, Mara</creatorcontrib><creatorcontrib>Felippe, Wilson</creatorcontrib><title>Influence of infected root dentin on the bond strength of a self-adhesive resin cement</title><title>Contemporary clinical dentistry</title><addtitle>Contemp Clin Dent</addtitle><description>Aim: The aim of this study was to determine the bond strength (BS) of a self-adhesive resin cement to the contaminated root dentin. Materials and Methods: The crown and apical third of twenty single-rooted teeth were removed. The root canals were flared and 1-mm-thick root sections were obtained. The sections were rinsed, dried, and sterilized. The control group (n=20) was composed of one section of each third, which remained immersed in sterile trypticase soy broth (TSB) for 2 months. The other sections comprised the experimental group (n = 40) and were immersed in a suspension of Enterococcus faecalis. The culture medium was changed at every 4 days for 2 months. The sections were rinsed with distilled water, dried, and the root canal space was fi lled with the self-adhesive resin cement RelyX™ U200. After 24 h, the push-out test was performed and the types of interface failure were observed on a stereo microscope. Statistical Analysis: Data were statistically analyzed by the nonparametric Mann-Whitney test (α=5%). Results: A significant reduction was observed in the BS of resin cement to the contaminated dentin compared to the healthy dentin, for both thirds analyzed (P &lt; 0.05). The BS was signifi cantly greater at the cervical third compared to the middle third for specimens in the experimental group (P &lt; 0.05). Adhesive and mixed failures were observed more frequently in specimens contaminated with E. faecalis. 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Materials and Methods: The crown and apical third of twenty single-rooted teeth were removed. The root canals were flared and 1-mm-thick root sections were obtained. The sections were rinsed, dried, and sterilized. The control group (n=20) was composed of one section of each third, which remained immersed in sterile trypticase soy broth (TSB) for 2 months. The other sections comprised the experimental group (n = 40) and were immersed in a suspension of Enterococcus faecalis. The culture medium was changed at every 4 days for 2 months. The sections were rinsed with distilled water, dried, and the root canal space was fi lled with the self-adhesive resin cement RelyX™ U200. After 24 h, the push-out test was performed and the types of interface failure were observed on a stereo microscope. Statistical Analysis: Data were statistically analyzed by the nonparametric Mann-Whitney test (α=5%). Results: A significant reduction was observed in the BS of resin cement to the contaminated dentin compared to the healthy dentin, for both thirds analyzed (P &lt; 0.05). The BS was signifi cantly greater at the cervical third compared to the middle third for specimens in the experimental group (P &lt; 0.05). Adhesive and mixed failures were observed more frequently in specimens contaminated with E. faecalis. Conclusion: Bacterial contamination negatively infl uenced the BS of the self-adhesive resin cement to the root dentin, and there was a predominance of adhesive and mixed failures.</abstract><cop>India</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><pmid>29599579</pmid><doi>10.4103/ccd.ccd_683_17</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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ispartof Contemporary clinical dentistry, 2018-01, Vol.9 (1), p.26-30
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subjects Adhesion
Adhesive systems
Adhesives
Bond strength
Cement
Collagen
contaminated dentin
Contamination
Data processing
Dental cement
Dental crowns
Dentin
Dentistry
Enamel
Endodontics
Failure analysis
Microorganisms
Original
push-out test
Resins
Root canal therapy
Root canals
Statistical analysis
Teeth
title Influence of infected root dentin on the bond strength of a self-adhesive resin cement
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