Turning up the heat on essential E. coli genes

Temperature‐sensitive (TS) alleles create tunable thermoswitches to deplete essential cellular activities and are used to dissect gene function. In their recent study, Link and colleagues (Schramm et al 2023) use a CRISPR‐based approach to systematically create TS alleles across essential genes in E...

Full description

Saved in:
Bibliographic Details
Published in:Molecular systems biology 2023-10, Vol.19 (10), p.e11933-e11933
Main Authors: Kumar, Arun, Stirling, Peter C
Format: Article
Language:English
Subjects:
Alleles
Amino acids
CRISPR
Cyanobacteria
Design
E coli
Genes
Metabolism
Metabolites
Mutation
Organisms
Proteins
Synthetic biology
Temperature
Views
Yeast
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Temperature‐sensitive (TS) alleles create tunable thermoswitches to deplete essential cellular activities and are used to dissect gene function. In their recent study, Link and colleagues (Schramm et al 2023) use a CRISPR‐based approach to systematically create TS alleles across essential genes in E. coli .
ISSN:1744-4292
1744-4292
DOI:10.15252/msb.202311933