Correlation Between Serum 25-hydroxyvitamin D Content and Testosterone Content in Young Healthy Males: A Single Center Cross-Sectional Study

• Published in: The international journal of frontier sciences 2024-07, Vol.7 (1)
• Main Authors: Sara Khan, Sibgha Bashir, Ayesha Siddiqa, Rizwan Hafeez, Anum Iftikhar, Urwah Ehsan
• Format: Article
• Language: English
• Subjects: 25-Hydroxy Vitamen D; Total Testosterone; Young Healthy Males
• ISSN: 2618-0359; 2618-0367
• DOI: 10.37978/tijfs.v7i1.418

Objectives: The current study is conducted to compare serum 25-hydroxyvitamin D concentrations and total testosterone in young, physically fit adult males. Study Design: the study was based on cross sectional design. Study Settings: The research was conducted in Department of Pathology, Shahida Islam Medical and Dental College situated in District Lodhran between 1st July, 2023 and December, 2023. Methods:  This cross-sectional research, which took place at the Department of Pathology at Shahida Islam Medical and Dental College in Lodhran from July 1, 2023, to December 31, 2023, had 176 young male volunteers, all between the ages of 18 and 35. Each participant was given questionnaires to fill out on their prior medical history, food habits, and rigorous exercise levels. Every participant had their blood drawn following a 12-hour fast and a 24-hour period without physical exercise. Separating serum was done with a centrifuge. The blood concentration of LH, FSH, total testosterone, and 25-hydroxyvitamin D were measured by electrochemiluminescence (ECLIA). Results: A total of 176 individuals in good health were registered, with a mean age of 26.62±5.20 years. The presence of vitamin D of less than 10 ng/ml, between 10–20 ng/ml, and greater than 20 ng/ml were reported in 70 (39.8%), 76 (43.2%), and 30 (17.0%) individuals, accordingly. The mean hormone levels (TT, FSH, and LH) in the three 25(OH)D groups did not vary statistically. Our findings showed that there existed no statistically substantial relationship in the categories under study between 25(OH)D and LH, FSH, and TT. Conclusion: We found little variation in the condition of the 25(OH)D concentration and the average hormonal measurements (LH, FSH, and TT). Based on these data, we concluded that in young, healthy guys, there is no relationship between testosterone concentrations and deficient or inadequate 25(OH)D level.