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Long Non-Coding RNA lncWOX11a Suppresses Adventitious Root Formation of Poplar by Regulating the Expression of PeWOX11a
Long non-coding RNAs (lncRNAs), a class of poorly conserved transcripts without protein-encoding ability, are widely involved in plant organogenesis and stress responses by mediating the transmission and expression of genetic information at the transcriptional, posttranscriptional, and epigenetic le...
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Published in: | International journal of molecular sciences 2023-03, Vol.24 (6), p.5766 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Long non-coding RNAs (lncRNAs), a class of poorly conserved transcripts without protein-encoding ability, are widely involved in plant organogenesis and stress responses by mediating the transmission and expression of genetic information at the transcriptional, posttranscriptional, and epigenetic levels. Here, we cloned and characterized a novel lncRNA molecule through sequence alignment, Sanger sequencing, transient expression in protoplasts, and genetic transformation in poplar.
is a 215 bp transcript located on poplar chromosome 13, ~50 kbp upstream of
on the reverse strand, and the lncRNA may fold into a series of complex stem-loop structures. Despite the small open reading frame (sORF) of 51 bp within
, bioinformatics analysis and protoplast transfection revealed that
has no protein-coding ability. The overexpression of
led to a decrease in the quantity of adventitious roots on the cuttings of transgenic poplars. Further,
-regulatory module prediction and CRISPR/Cas9 knockout experiments with poplar protoplasts demonstrated that
acts as a negative regulator of adventitious rooting by downregulating the WUSCHEL-related homeobox gene
, which is supposed to activate adventitious root development in plants. Collectively, our findings imply that
is essential for modulating the formation and development of adventitious roots. |
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ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms24065766 |