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First European Erwinia amylovora Lytic Bacteriophage Cocktails Effective in the Host: Characterization and Prospects for Fire Blight Biocontrol
Fire blight, caused by the plant-pathogenic bacterium , is a highly contagious and difficult-to-control disease due to its efficient dissemination and survival and the scarcity of effective control methods. Copper and antibiotics are the most used treatments but pose environmental and human health r...
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| Published in: | Biology (Basel, Switzerland) Switzerland), 2024-03, Vol.13 (3), p.176 |
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| Main Authors: | , , , , , , |
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| container_title | Biology (Basel, Switzerland) |
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| creator | Biosca, Elena G Delgado Santander, Ricardo Morán, Félix Figàs-Segura, Àngela Vázquez, Rosa Català-Senent, José Francisco Álvarez, Belén |
| description | Fire blight, caused by the plant-pathogenic bacterium
, is a highly contagious and difficult-to-control disease due to its efficient dissemination and survival and the scarcity of effective control methods. Copper and antibiotics are the most used treatments but pose environmental and human health risks. Bacteriophages (phages) constitute an ecological, safe, and sustainable fire blight control alternative. The goal of this study was to search for specific
phages from plant material, soil, and water samples in Mediterranean environments. A collection of phages able to specifically infect and lyse
strains was generated from former fire blight-affected orchards in Eastern Spain. Following
characterization, assays in immature fruit revealed that preventively applying some of the phages or their combinations delayed the onset of fire blight symptoms and reduced the disease's severity, suggesting their biocontrol potential in Spain and other countries. The morphological and molecular characterization of the selected
phages classified them as members of the class
(former
family) and genus
. This study reveals Mediterranean settings as plausible sources of
-specific bacteriophages and provides the first effective European phage cocktails in plant material for the development of sustainable fire blight management measures. |
| doi_str_mv | 10.3390/biology13030176 |
| format | article |
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, is a highly contagious and difficult-to-control disease due to its efficient dissemination and survival and the scarcity of effective control methods. Copper and antibiotics are the most used treatments but pose environmental and human health risks. Bacteriophages (phages) constitute an ecological, safe, and sustainable fire blight control alternative. The goal of this study was to search for specific
phages from plant material, soil, and water samples in Mediterranean environments. A collection of phages able to specifically infect and lyse
strains was generated from former fire blight-affected orchards in Eastern Spain. Following
characterization, assays in immature fruit revealed that preventively applying some of the phages or their combinations delayed the onset of fire blight symptoms and reduced the disease's severity, suggesting their biocontrol potential in Spain and other countries. The morphological and molecular characterization of the selected
phages classified them as members of the class
(former
family) and genus
. This study reveals Mediterranean settings as plausible sources of
-specific bacteriophages and provides the first effective European phage cocktails in plant material for the development of sustainable fire blight management measures.</description><identifier>ISSN: 2079-7737</identifier><identifier>EISSN: 2079-7737</identifier><identifier>DOI: 10.3390/biology13030176</identifier><identifier>PMID: 38534446</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antibiotics ; Bacteria ; Biological control ; Blight ; Chemical control ; Copper ; Disease control ; Disease prevention ; Economic importance ; environmental samples ; Erwinia amylovora ; Flowers & plants ; Fruit trees ; Fruits ; lysis ; Mediterranean environments ; Pathogens ; Phages ; phytopathogenic bacteria ; Plant diseases ; Public health ; Quarantine ; specificity</subject><ispartof>Biology (Basel, Switzerland), 2024-03, Vol.13 (3), p.176</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the authors. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c442t-daf267d82c9852405b47809e5bb491be72f85f6ce75f19f396909ddd8b1bf7aa3</cites><orcidid>0000-0002-8590-6180 ; 0000-0002-5892-7437 ; 0000-0002-1309-2632 ; 0000-0001-9300-3953 ; 0000-0001-5850-1527</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2997631385/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2997631385?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25752,27923,27924,37011,37012,44589,53790,53792,74897</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38534446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Biosca, Elena G</creatorcontrib><creatorcontrib>Delgado Santander, Ricardo</creatorcontrib><creatorcontrib>Morán, Félix</creatorcontrib><creatorcontrib>Figàs-Segura, Àngela</creatorcontrib><creatorcontrib>Vázquez, Rosa</creatorcontrib><creatorcontrib>Català-Senent, José Francisco</creatorcontrib><creatorcontrib>Álvarez, Belén</creatorcontrib><title>First European Erwinia amylovora Lytic Bacteriophage Cocktails Effective in the Host: Characterization and Prospects for Fire Blight Biocontrol</title><title>Biology (Basel, Switzerland)</title><addtitle>Biology (Basel)</addtitle><description>Fire blight, caused by the plant-pathogenic bacterium
, is a highly contagious and difficult-to-control disease due to its efficient dissemination and survival and the scarcity of effective control methods. Copper and antibiotics are the most used treatments but pose environmental and human health risks. Bacteriophages (phages) constitute an ecological, safe, and sustainable fire blight control alternative. The goal of this study was to search for specific
phages from plant material, soil, and water samples in Mediterranean environments. A collection of phages able to specifically infect and lyse
strains was generated from former fire blight-affected orchards in Eastern Spain. Following
characterization, assays in immature fruit revealed that preventively applying some of the phages or their combinations delayed the onset of fire blight symptoms and reduced the disease's severity, suggesting their biocontrol potential in Spain and other countries. The morphological and molecular characterization of the selected
phages classified them as members of the class
(former
family) and genus
. This study reveals Mediterranean settings as plausible sources of
-specific bacteriophages and provides the first effective European phage cocktails in plant material for the development of sustainable fire blight management measures.</description><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Biological control</subject><subject>Blight</subject><subject>Chemical control</subject><subject>Copper</subject><subject>Disease control</subject><subject>Disease prevention</subject><subject>Economic importance</subject><subject>environmental samples</subject><subject>Erwinia amylovora</subject><subject>Flowers & plants</subject><subject>Fruit trees</subject><subject>Fruits</subject><subject>lysis</subject><subject>Mediterranean environments</subject><subject>Pathogens</subject><subject>Phages</subject><subject>phytopathogenic bacteria</subject><subject>Plant diseases</subject><subject>Public health</subject><subject>Quarantine</subject><subject>specificity</subject><issn>2079-7737</issn><issn>2079-7737</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdksFuEzEQhlcIRKvQMzdkiQuXUHvttddcEIlSWqkSHOBszXrtxGGzE2wnKLwEr4xDStXWF1vjf77xjP-qes3oe841vewCDrg8ME45ZUo-q85rqvRUKa6ePzifVRcprWlZitaSy5fVGW8bLoSQ59WfqxBTJotdxK2DkSzirzAGILA5DLjHCOT2kIMlM7DZxYDbFSwdmaP9kSEMiSy8dzaHvSNhJHnlyDWm_IHMVxBPGb8hBxwJjD35GjFtizoRj5GUwo7MhrBcZTILaHHMEYdX1QsPQ3IXd_uk-n61-Da_nt5--Xwz_3Q7tULUedqDr6Xq29rqtqkFbTqhWqpd03VCs86p2reNl9apxjPtuZaa6r7v2451XgHwSXVz4vYIa7ONYQPxYBCC-RfAuDQQS-ODM86ypgPW0lYw4UXbCWu14rIRlHIvdGF9PLG2u27jeutKJzA8gj6-GcPKLHFvGNVSKSkK4d0dIeLPnUvZbEKybhhgdLhLhpdKggutjtK3T6Rr3MWxzMrUWivJ2fFzJ9XlSWXLzFN0_v41jJqjecwT85SMNw-buNf_twr_C9JGw20</recordid><startdate>20240308</startdate><enddate>20240308</enddate><creator>Biosca, Elena G</creator><creator>Delgado Santander, Ricardo</creator><creator>Morán, Félix</creator><creator>Figàs-Segura, Àngela</creator><creator>Vázquez, Rosa</creator><creator>Català-Senent, José Francisco</creator><creator>Álvarez, Belén</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-8590-6180</orcidid><orcidid>https://orcid.org/0000-0002-5892-7437</orcidid><orcidid>https://orcid.org/0000-0002-1309-2632</orcidid><orcidid>https://orcid.org/0000-0001-9300-3953</orcidid><orcidid>https://orcid.org/0000-0001-5850-1527</orcidid></search><sort><creationdate>20240308</creationdate><title>First European Erwinia amylovora Lytic Bacteriophage Cocktails Effective in the Host: Characterization and Prospects for Fire Blight Biocontrol</title><author>Biosca, Elena G ; 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, is a highly contagious and difficult-to-control disease due to its efficient dissemination and survival and the scarcity of effective control methods. Copper and antibiotics are the most used treatments but pose environmental and human health risks. Bacteriophages (phages) constitute an ecological, safe, and sustainable fire blight control alternative. The goal of this study was to search for specific
phages from plant material, soil, and water samples in Mediterranean environments. A collection of phages able to specifically infect and lyse
strains was generated from former fire blight-affected orchards in Eastern Spain. Following
characterization, assays in immature fruit revealed that preventively applying some of the phages or their combinations delayed the onset of fire blight symptoms and reduced the disease's severity, suggesting their biocontrol potential in Spain and other countries. The morphological and molecular characterization of the selected
phages classified them as members of the class
(former
family) and genus
. This study reveals Mediterranean settings as plausible sources of
-specific bacteriophages and provides the first effective European phage cocktails in plant material for the development of sustainable fire blight management measures.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38534446</pmid><doi>10.3390/biology13030176</doi><orcidid>https://orcid.org/0000-0002-8590-6180</orcidid><orcidid>https://orcid.org/0000-0002-5892-7437</orcidid><orcidid>https://orcid.org/0000-0002-1309-2632</orcidid><orcidid>https://orcid.org/0000-0001-9300-3953</orcidid><orcidid>https://orcid.org/0000-0001-5850-1527</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Biology (Basel, Switzerland), 2024-03, Vol.13 (3), p.176 |
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| source | Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central |
| subjects | Antibiotics Bacteria Biological control Blight Chemical control Copper Disease control Disease prevention Economic importance environmental samples Erwinia amylovora Flowers & plants Fruit trees Fruits lysis Mediterranean environments Pathogens Phages phytopathogenic bacteria Plant diseases Public health Quarantine specificity |
| title | First European Erwinia amylovora Lytic Bacteriophage Cocktails Effective in the Host: Characterization and Prospects for Fire Blight Biocontrol |
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