Calciprotein particles induce arterial stiffening ex vivo and impair vascular cell function

• Published in: Communications biology 2024-10, Vol.7 (1), p.1241-13, Article 1241
• Main Authors: Neutel, Cédric H. G., Wesley, Callan D., van Loo, Cindy, Civati, Céline, Mertens, Freke, Zurek, Michelle, Verhulst, Anja, Pintelon, Isabel, De Vos, Winnok H., Spronck, Bart, Roth, Lynn, De Meyer, Guido R. Y., Martinet, Wim, Guns, Pieter-Jan
• Format: Article
• Language: English
• Subjects: 13/1; 13/106; 14/63; 631/114/2397; 631/443/592/2193; 631/443/810; 631/80/304; 64/60; 82/51; Animals; Aorta; Aorta, Thoracic - metabolism; Biomedical and Life Sciences; Calcium buffering; Coronary vessels; Extracellular matrix; Glycosaminoglycans; Glycosaminoglycans - metabolism; Humans; Life Sciences; Male; Mineralization; Muscle, Smooth, Vascular - metabolism; Myocytes, Smooth Muscle - metabolism; Rats; Smooth muscle; Thorax; Vascular Stiffness; Vasodilation
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-024-06895-y

Calciprotein particles (CPPs) are an endogenous buffering system, clearing excessive amounts of Ca 2+ and PO 4 3- from the circulation and thereby preventing ectopic mineralization. CPPs circulate as primary CPPs (CPP1), which are small spherical colloidal particles, and can aggregate to form large, crystalline, secondary CPPs (CPP2). Even though it has been reported that CPPs are toxic to vascular smooth muscle cells (VSMC) in vitro, their effect(s) on the vasculature remain unclear. Here we have shown that CPP1, but not CPP2, increased arterial stiffness ex vivo. Interestingly, the effects were more pronounced in the abdominal infrarenal aorta compared to the thoracic descending aorta. Further, we demonstrated that CPP1 affected both endothelial and VSMC function, impairing vasorelaxation and contraction respectively. Concomitantly, arterial glycosaminoglycan accumulation was observed as well, which is indicative of an increased extracellular matrix stiffness. However, these effects were not observed in vivo. Hence, we concluded that CPP1 can induce vascular dysfunction. Calciprotein particles (CPPs) help clear excess Ca 2+ and PO 4 3- to prevent mineralization. However, this study unveils the pathophysiological role of CPPs in arterial stiffness and impaired smooth muscle cell function ex vivo.