The Roles of Transient Receptor Potential Vanilloid 1 and 4 in Pneumococcal Nasal Colonization and Subsequent Development of Invasive Disease

• Published in: Frontiers in immunology 2021-11, Vol.12, p.732029-732029
• Main Authors: Kono, Masamitsu, Nanushaj, Denisa, Sakatani, Hideki, Murakami, Daichi, Hijiya, Masayoshi, Kinoshita, Tetsuya, Shiga, Tatsuya, Kaneko, Fumie, Enomoto, Keisuke, Sugita, Gen, Miyajima, Masayasu, Okada, Yuka, Saika, Shizuya, Hotomi, Muneki
• Format: Article
• Language: English
• Subjects: Animals; Coinfection; Cytokines - metabolism; Disease Models, Animal; Host-Pathogen Interactions; Immunology; Inflammation Mediators - metabolism; Influenza A Virus, H3N2 Subtype - pathogenicity; Mice; Mice, Inbred C57BL; Mice, Knockout; mouse model; nasal colonization; Nasal Mucosa - immunology; Nasal Mucosa - metabolism; Nasal Mucosa - microbiology; Nasal Mucosa - virology; Neutrophils - immunology; Neutrophils - metabolism; Neutrophils - microbiology; Orthomyxoviridae Infections - immunology; Orthomyxoviridae Infections - metabolism; Orthomyxoviridae Infections - microbiology; Phagocytosis; Pneumococcal Infections - genetics; Pneumococcal Infections - immunology; Pneumococcal Infections - metabolism; Pneumococcal Infections - microbiology; pneumonia; sepsis; Signal Transduction; Streptococcus pneumoniae (pneumococcus); Streptococcus pneumoniae - immunology; Streptococcus pneumoniae - pathogenicity; transient receptor potential (TRP); TRPV Cation Channels - genetics; TRPV Cation Channels - metabolism; Virulence
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2021.732029

Transient receptor potential (TRP) channels, neuronal stimulations widely known to be associated with thermal responses, pain induction, and osmoregulation, have been shown in recent studies to have underlying mechanisms associated with inflammatory responses. The role of TRP channels on inflammatory milieu during bacterial infections has been widely demonstrated. It may vary among types of channels/pathogens, however, and it is not known how TRP channels function during pneumococcal infections. can cause severe infections such as pneumonia, bacteremia, and meningitis, with systemic inflammatory responses. This study examines the role of TRP channels (TRPV1 and TRPV4) for pneumococcal nasal colonization and subsequent development of invasive pneumococcal disease in a mouse model. Both TRPV1 and TRPV4 channels were shown to be related to regulation of the development of pneumococcal diseases. In particular, the influx of neutrophils (polymorphonuclear cells) in the nasal cavity and the bactericidal activity were significantly suppressed among TRPV4 knockout mice. This may lead to severe pneumococcal pneumonia, resulting in dissemination of the bacteria to various organs and causing high mortality during influenza virus coinfection. Regulating host immune responses by TRP channels could be a novel strategy against pathogenic microorganisms causing strong local/systemic inflammation.