Downregulated XPA promotes carcinogenesis of bladder cancer via impairment of DNA repair

• Published in: Tumor biology 2017-02, Vol.39 (2), p.1010428317691679-1010428317691679
• Main Authors: Zhi, Yi, Ji, Huixiang, Pan, Jinhong, He, Peng, Zhou, Xiaozhou, Zhang, Heng, Zhou, Zhansong, Chen, Zhiwen
• Format: Article
• Language: English
• Subjects: Aged; Anti-oncogenes; Bladder cancer; Cancer; Carcinogenesis; Carcinogenesis - genetics; CDC2 Protein Kinase; Cell cycle; Cell Cycle - genetics; Cell Line, Tumor; Chromosome Aberrations; Comet Assay; Cyclin B - genetics; Cyclin B - metabolism; Cyclin E - genetics; Cyclin E - metabolism; Cyclin-Dependent Kinase 2 - genetics; Cyclin-Dependent Kinase 2 - metabolism; Cyclin-dependent kinases; Cyclin-Dependent Kinases - genetics; Cyclin-Dependent Kinases - metabolism; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded; DNA damage; DNA Repair; Double-strand break repair; Down-Regulation; Genes; Histones - genetics; Histones - metabolism; Humans; Impairment; Kinases; Lung cancer; Male; Nucleotide excision repair; Polymerase chain reaction; Proteins; Roles; Tumorigenesis; Urinary bladder; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - metabolism; Xeroderma Pigmentosum Group A Protein - genetics; Xeroderma Pigmentosum Group A Protein - metabolism
• ISSN: 1010-4283; 1423-0380
• DOI: 10.1177/1010428317691679

Bladder cancer is the most common malignant tumor of urinary system, largely resulting from failure of repair of DNA damage to the environmental insults. The function of XPA in nucleotide excision repair pathway has been well documented. However, participation of XPA in the repair of DNA double-strand break remains unknown. Here, we reported that bladder cancer expressed low XPA levels compared to adjacent non-tumor bladder tissue, and this phenotype was closely associated with chromosomal aberrations. Moreover, downregulated XPA appeared to increase incidence of chromosome aberration. XPA reduction increased cell viability of a bladder cancer cell line RT4, while XPA re-expression decreased the cell viability of RT4 cells. Since high mutation frequency is the basis of mutations of oncogenes and anti-oncogenes, and may be the essence of bladder cancer susceptibility, our study suggests that downregulated XPA may promote carcinogenesis of bladder cancer via impairment of DNA repair.