Phenothiazines Rapidly Induce Laccase Expression and Lignin-Degrading Properties in the White-Rot Fungus Phlebia radiata

is a widespread white-rot basidiomycete fungus with significance in diverse biotechnological applications due to its ability to degrade aromatic compounds, xenobiotics, and lignin using an assortment of oxidative enzymes including laccase. In this work, a chemical screen with 480 conditions was cond...

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Bibliographic Details
Published in:Journal of fungi (Basel) 2023-03, Vol.9 (3), p.371
Main Authors: Hirakawa, Matthew P, Rodriguez, Alberto, Tran-Gyamfi, Mary B, Light, Yooli K, Martinez, Salvador, Diamond-Pott, Henry, Simmons, Blake A, Sale, Kenneth L
Format: Article
Language:English
Subjects:
Aromatic compounds
BASIC BIOLOGICAL SCIENCES
Bioremediation
Carbon
Enzymatic activity
Enzymes
Experiments
Fungi
Gene expression
Genes
Genotype & phenotype
Glucose
Laccase
Lignin
Metabolism
Nitrogen
Phenolic compounds
Phenothiazine
Phlebia radiata
Promethazine
Transcriptomics
White rot
white-rot fungi
Xenobiotics
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Summary:is a widespread white-rot basidiomycete fungus with significance in diverse biotechnological applications due to its ability to degrade aromatic compounds, xenobiotics, and lignin using an assortment of oxidative enzymes including laccase. In this work, a chemical screen with 480 conditions was conducted to identify chemical inducers of laccase expression in . Among the chemicals tested, phenothiazines were observed to induce laccase activity in with promethazine being the strongest laccase inducer of the phenothiazine-derived compounds examined. Secretomes produced by promethazine-treated exhibited increased laccase protein abundance, increased enzymatic activity, and an enhanced ability to degrade phenolic model lignin compounds. Transcriptomics analyses revealed that promethazine rapidly induced the expression of genes encoding lignin-degrading enzymes, including laccase and various oxidoreductases, showing that the increased laccase activity was due to increased laccase gene expression. Finally, the generality of promethazine as an inducer of laccases in fungi was demonstrated by showing that promethazine treatment also increased laccase activity in other relevant fungal species with known lignin conversion capabilities including and
ISSN:2309-608X
2309-608X
DOI:10.3390/jof9030371