Combined low-dose LiCl and LY294002 for the treatment of osteoporosis in ovariectomized rats

To provide a low-toxicity and high-efficacy clinical treatment for osteoporosis via a novel combination of LiCl and LY294002. The protein levels of p-AKT, AKT, p-GSK3β, GSK3β, β-catenin, p-β-catenin, and NFATC1 were measured in osteoblasts and osteoclasts by Western blot. ALP activity and TRACP acti...

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Bibliographic Details
Published in:Journal of orthopaedic surgery and research 2019-06, Vol.14 (1), p.177-177, Article 177
Main Authors: Bai, Jianhai, Xu, Yier, Dieo, Yan, Sun, Guicai
Format: Article
Language:English
Subjects:
Abdomen
Acid phosphatase (tartrate-resistant)
AKT protein
Animals
Apoptosis
Chromones - administration & dosage
Collagen
Computed tomography
Disease
Dosage and administration
Dose-Response Relationship, Drug
Drug dosages
Drug therapy
Drug Therapy, Combination
Enzyme-linked immunosorbent assay
Experiments
Female
Femur
Gene expression
Health aspects
Imaging, Three-Dimensional - methods
Kinases
Laboratory animals
Ligands
Lithium chloride
Lithium Chloride - administration & dosage
Lithium compounds
LY294002
Medical research
Metabolism
Morpholines - administration & dosage
NFATC1
Orthopedics
Osteoblastogenesis
Osteoblasts
Osteoclastogenesis
Osteoclasts
Osteoporosis
Osteoporosis - diagnostic imaging
Osteoporosis - drug therapy
Osteoporosis - etiology
Ovariectomy
Ovariectomy - adverse effects
Rats
Rats, Sprague-Dawley
Rodents
Signal transduction
Stem cells
Surgery
Testing
Toxicity
Treatment Outcome
β-Catenin
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Summary:To provide a low-toxicity and high-efficacy clinical treatment for osteoporosis via a novel combination of LiCl and LY294002. The protein levels of p-AKT, AKT, p-GSK3β, GSK3β, β-catenin, p-β-catenin, and NFATC1 were measured in osteoblasts and osteoclasts by Western blot. ALP activity and TRACP activity were measured using the corresponding kit. The levels of BALP, PINP, CTX, and TRACP-5b were determined in accordance with the requirements of the ELISA kits. Microstructural analysis was performed on the left distal femur using microcomputed tomography. Treatment with the combination of LiCl and LY294002 led to a markedly increased osteoblast activity but significantly decreased osteoclast differentiation and bone absorption capacity compared with the treatment with LiCl or LY294002 alone (P 
ISSN:1749-799X
1749-799X
DOI:10.1186/s13018-019-1210-1