Occurrence of Plasmid-Mediated Quinolone Resistance and Carbapenemase-Encoding Genes in Pseudomonas aeruginosa Isolates from Nosocomial Patients in Aguascalientes, Mexico

• Published in: Pathogens (Basel) 2024-11, Vol.13 (11), p.992
• Main Authors: Tapia-Cornejo, Ana S, Ramírez-Castillo, Flor Y, Guerrero-Barrera, Alma L, Guillen-Padilla, Diana E, Arreola-Guerra, José M, González-Gámez, Mario, Avelar-González, Francisco J, Loera-Muro, Abraham, Hernández-Cuellar, Eduardo, Ramos-Medellín, Carmen L, Adame-Álvarez, Cesar, García-Romo, Ricardo, Galindo-Guerrero, Fabiola, Moreno-Flores, Adriana C
• Format: Article
• Language: English
• Subjects: Analysis; Antibacterial agents; Antibiotics; Antimicrobial agents; Bacteria; Carbapenemase; carbapenemase-encoding genes; Carbapenems; Ceftriaxone; China; Drug resistance; Genes; Health aspects; Hospitals; Infection; Iran; mcr-1; Mexico; Morbidity; Mortality; Multidrug resistance; Nosocomial infection; Nosocomial infections; Observational studies; Oxalic acid; Patients; Plasmid-Mediated Quinolone Resistance (PMQR) genes; Polymerase chain reaction; Pseudomonas aeruginosa; Quinolones; Strain analysis; Tetracycline; Tetracyclines; Tigecycline; United States
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens13110992

is a leading cause of healthcare-associated infections, which are related to substantial morbidity and mortality. The incidence of Plasmid-Mediated Quinolone Resistance (PMQR) determinants has been previously reported in this bacterium. However, there is limited information regarding the presence of PMQR and carbapenemase-encoding genes simultaneously. This study aims to analyze the prevalence of these determinants on strain isolated from clinical patients in the State of Aguascalientes, Mexico. Fifty-two isolates from nosocomial patients were collected from Centenario Hospital Miguel Hidalgo. This is a retrospective observational study conducted at a single center. Antibiotic susceptibility was tested using the Vitek-2 system. Only carbapenem-resistant isolates were included in this study. Carbapenemase-encoding genes and PMQR determinants were screened by polymerase chain reaction (PCR). Resistance rates of 100% were found on tigecycline and ceftriaxone. Of the 52 isolates, 34.6% were positive for the genes, 46.2% for the gene, and 25% for the gene. The most frequent carbapenemase genes found in the samples were (42.3%), (15.4%), and (15.4%). co-carrying was detected in 21.1% of the isolates, co-carrying in 11.5%, co-carrying in 3.8%, and co-carrying in 5.8%. Systematic surveillance to detect carbapenemase-encoding genes and PMQR determinants, and rational prescription using the last-line drugs could help in preventing the dissemination of multidrug-resistant determinants.