Ishophloroglucin A, Isolated from Ishige okamurae , Alleviates Dexamethasone-Induced Muscle Atrophy through Muscle Protein Metabolism In Vivo

• Published in: Marine drugs 2022-04, Vol.20 (5), p.280
• Main Authors: Yang, Hye-Won, Oh, Seyeon, Chung, Dong-Min, Seo, Minyoung, Park, Shin Jae, Jeon, You-Jin, Byun, Kyunghee, Ryu, BoMi
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 3-kinase; Adenosine; AKT protein; Animal tissues; Animals; Atrophy; Benzofurans; Complex Mixtures - pharmacology; Complex Mixtures - therapeutic use; Dexamethasone; Dexamethasone - adverse effects; Dioxins; Dual energy X-ray absorptiometry; Grip strength; Ishige okamurae; Ishophloroglucin A; Metabolism; Mice; Morphology; mRNA; muscle atrophy; Muscle function; muscle growth; muscle protein metabolism; Muscle Proteins - metabolism; Muscle recovery; Muscle strength; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscles; Muscular Atrophy - chemically induced; Muscular Atrophy - drug therapy; Muscular Atrophy - metabolism; Musculoskeletal system; Myostatin; Oral administration; Phaeophyceae - metabolism; Physiology; Protein biosynthesis; Protein metabolism; Protein synthesis; Protein turnover; Proteins; Receptors; RNA, Messenger - metabolism; Soleus muscle; Steroids; Supplements; Transient receptor potential proteins; Tumor necrosis factor-TNF; Ubiquitin; Ubiquitin-protein ligase
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md20050280

The in vitro capacity of extract (IO) to improve impaired muscle function has been previously examined. However, the mechanism underlying IO-mediated muscle protein metabolism and the role of its component, Ishophloroglucin A (IPA), in mice with dexamethasone (Dexa)-induced muscle atrophy remains unknown. In the present study, we evaluated the effect of IO and IPA supplementation on Dexa-induced muscle atrophy by assessing muscle protein metabolism in gastrocnemius and soleus muscles of mice. IO and IPA supplementation improved the Dexa-induced decrease in muscle weight and width, leading to enhanced grip strength. In addition, IO and IPA supplementation regulated impaired protein synthesis (PI3K and Akt) or degradation (muscle-specific ubiquitin ligase muscle RING finger and atrogin-1) by modulating mRNA levels in gastrocnemius and soleus muscles. Additionally, IO and IPA upregulated mRNA levels associated with muscle growth activation (transient receptor potential vanilloid type 4 and adenosine A1 receptor) or inhibition (myostatin and sirtuin 1) in gastrocnemius and soleus muscle tissues of Dexa-induced mice. Collectively, these results suggest that IO and IO-derived IPA can regulate muscle growth through muscle protein metabolism in Dexa-induced muscle atrophy.