Patterns of Genetic Diversity and Mating Systems in a Mass-Reared Black Soldier Fly Colony

• Published in: Insects (Basel, Switzerland) Switzerland), 2021-05, Vol.12 (6), p.480
• Main Authors: Hoffmann, Lelanie, Hull, Kelvin L., Bierman, Anandi, Badenhorst, Rozane, Bester-van der Merwe, Aletta E., Rhode, Clint
• Format: Article
• Language: English
• Subjects: Agricultural production; Animal behavior; Animal husbandry; Assortative mating; Bioremediation; Breeding; Climate change; Colonies; Divergence; Domestication; Farming; Fish meal; Food production; Generations; genetic differentiation; Genetic diversity; Genetic effects; Genetic markers; Genetic relationship; Hermetia illucens; Homozygosity; Human populations; Inbreeding; insect culture; Insects; Larvae; Life history; Livestock; Livestock farming; Males; Mass rearing; Mate selection; Mating; microsatellite markers; Microsatellites; multiple paternity; Natural resources; Organic wastes; Parameter estimation; Paternity; Polyandry; Population; Population dynamics; Population genetics; Populations; Productivity; Reproductive behavior; Sustainability; Sustainable agriculture; Trends
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects12060480

The black soldier fly (BSF), Hermetia illucens, is a promising candidate for the emerging insect farming industry with favourable characteristics for both bioremediation and production of animal delivered nutritive and industrial compounds. The genetic management of commercial colonies will become increasingly important for the sustainability of the industry. However, r-selected life history traits of insects pose challenges to conventional animal husbandry and breeding approaches. In this study, the long-term genetic effects of mass-rearing were evaluated as well as mating systems in the species to establish factors that might influence genetic diversity, and by implication fitness and productivity in commercial colonies. Population genetic parameters, based on microsatellite markers, were estimated and compared amongst two temporal wild sampling populations and four generations (F28, F48, F52, and F62) of a mass-reared colony. Furthermore, genetic relationships amongst mate pairs were evaluated and parentage analysis was performed to determine the oc-currence of preferential mate choice and multiple paternity. The mass-reared colony showed a reduction in genetic diversity and evidence for inbreeding with significant successive generational genetic differentiation from the wild progenitor population. Population-level analysis also gave the first tentative evidence of positive assortative mating and genetic polyandry in BSF. The homoge-neity of the mass-reared colony seems to result from a dual action caused by small effective popu-lation size and increased homozygosity due to positive assortative mating. However, the high ge-netic diversity in the wild and a polyandrous mating system might suggest the possible restoration of diversity in mass-reared colonies through augmentation with the wild population.