Tannic acid elicits differential gene regulation in prostate cancer apoptosis

• Published in: Acta pharmaceutica (Zagreb, Croatia) Croatia), 2024-09, Vol.74 (3), p.539-550
• Main Authors: Kandir, Sinan, Karakurt, Sevtap, Gökçek-Saraç, Çiğdem, Karakurt, Serdar
• Format: Article
• Language: English
• Subjects: Acids; Apoptosis; Apoptosis - drug effects; Bcl-10 protein; Bcl-2 protein; BNIP3 protein; CD40 antigen; Cell death; Cell growth; Cell Line, Tumor; Cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; Cytotoxicity; Down-Regulation - drug effects; Gadd45A protein; Gene expression; Gene Expression Regulation, Neoplastic - drug effects; Gene regulation; Humans; Male; Medical innovations; MyD88 protein; p53 Protein; PC-3 Cells; Polyphenols; Prostate cancer; Prostate carcinoma; Prostatic Neoplasms - drug therapy; Prostatic Neoplasms - genetics; Prostatic Neoplasms - pathology; Public health; selective cytotoxicity; Tannic acid; Tannins - pharmacology; TRAF2 protein; Tumor cell lines
• ISSN: 1330-0075; 1846-9558; 1846-9558
• DOI: 10.2478/acph-2024-0020

Prostate cancer is a significant global health concern that requires innovative therapeutic investigations. Here, the potential anticancer properties of tannic acid were evaluated by examining its effects on apoptosis in prostate cancer cell lines. PC-3 and LnCaP prostate adeno carcinoma cells, along with PNT1A prostate control cells, were cultured and divided into untreated and tannic acid-treated groups. Cell proliferation, cytotoxicity, and effects of tannic acid on the cell death mechanism were evaluated. mRNA expression levels of 84 genes were explored in cells following tannic acid treatment. Notably, tannic acid-induced down-regulation of several pro-survival genes, including and in both cell lines. Moreover, tannic acid treatment led to the up-regulation of various pro-apoptotic genes, such as in both PC-3 and LnCap cells. These findings highlight tannic acid’s ability to induce apoptosis in prostate cancer cells through pro-apoptotic pathways. This study concludes that tannic acid selectively inhibits prostate cancer cell growth.