Arabidopsis Chloroplast protein for Growth and Fertility1 (CGF1) and CGF2 are essential for chloroplast development and female gametogenesis

• Published in: BMC plant biology 2020-04, Vol.20 (1), p.172-172, Article 172
• Main Authors: Zhu, Rui-Min, Chai, Sen, Zhang, Zhuang-Zhuang, Ma, Chang-Le, Zhang, Yan, Li, Sha
• Format: Article
• Language: English
• Subjects: Amino acids; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biosynthesis; Cell death; Cell size; Chloroplast; Chloroplast Proteins - genetics; Chloroplast Proteins - metabolism; Chloroplasts; Chloroplasts - metabolism; CRISPR; Cyanobacteria; Defects; Developmental stages; Dwarfism; Embryogenesis; Embryonic growth stage; Embryos; Environmental effects; Fertility; Gametogenesis; Gametogenesis, Plant - genetics; Genetic aspects; Genomes; Genomics; Growth; Integrity; Membrane proteins; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Mutants; Mutation; Organelles; Ovule development; Peptides; Physiology; Plant cells; Plastid; Plastids; Proteins; Roles; Seeds; Sucrose; Sugar; Transgenic plants
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-020-02393-5

Chloroplasts are essential organelles of plant cells for not only being the energy factory but also making plant cells adaptable to different environmental stimuli. The nuclear genome encodes most of the chloroplast proteins, among which a large percentage of membrane proteins have yet to be functionally characterized. We report here functional characterization of two nuclear-encoded chloroplast proteins, Chloroplast protein for Growth and Fertility (CGF1) and CGF2. CGF1 and CGF2 are expressed in diverse tissues and developmental stages. Proteins they encode are associated with chloroplasts through a N-terminal chloroplast-targeting signal in green tissues but also located at plastids in roots and seeds. Mutants of CGF1 and CGF2 generated by CRISPR/Cas9 exhibited vegetative defects, including reduced leaf size, dwarfism, and abnormal cell death. CGF1 and CGF2 redundantly mediate female gametogenesis, likely by securing local energy supply. Indeed, mutations of both genes impaired chloroplast integrity whereas exogenous sucrose rescued the growth defects of the CGF double mutant. This study reports that two nuclear-encoded chloroplast proteins, Chloroplast protein for Growth and Fertility (CGF1) and CGF2, play important roles in vegetative growth, in female gametogenesis, and in embryogenesis likely by mediating chloroplast integrity and development.