Potential anti-HIV and antitrypanosomal components revealed in Sorindeia nitidula via LC-ESI-QTOF-MS/MS

• Published in: Scientific reports 2024-08, Vol.14 (1), p.18211-13, Article 18211
• Main Authors: Ebede, Guy Roland, Okoro, Emeka Emea, Ngo Mbing, Josephine, Diboue Betote, Patrick Herve, Pegnyemb, Dieudonne Emmanuel, Choudhary, Muhammad Iqbal, Siwe-Noundou, Xavier, Ndongo, Joseph Thierry
• Format: Article
• Language: English
• Subjects: 631/45; 639/638; Acetic acid; Anti-HIV Agents - chemistry; Anti-HIV Agents - pharmacology; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Bioactive compounds; Chemicals; Chemistry; Chromatography, Liquid - methods; Cytotoxicity; Ethyl acetate; Flavonoids; Forests; HeLa Cells; Herbal medicine; HIV; Human immunodeficiency virus; Humanities and Social Sciences; Humans; Influenza; Information processing; Metabolites; multidisciplinary; Parasites; Pharmaceuticals; Phenolic compounds; Phenols; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyphenols; Protozoa; Science; Science (multidisciplinary); Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry - methods; Trypanocidal Agents - chemistry; Trypanocidal Agents - pharmacology; Tumor cell lines; Vaccines
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-68364-0

Sorindeia nitidula (Anacardiaceae) is used by traditional practitioners to treat influenza illnesses with cephalgia and febrile aches. However, the potential active ingredients for its remarkable antioxidant, anti-HIV and antitrypanosomal activities remain unexplored. The present study aims to evaluate the antioxidant, anti-HIV and antitrypanosomal activities of the ethyl acetate extract of S. nitidula (SN) in order to screen out the bioactive compounds and to analyze their possible mechanisms of action. Overall, 21 phenolic compounds were annotated, by using the MS and MS/MS information provided by the QTOF-MS. In vitro assays on the extract revealed potent antioxidant (IC 50  = 0.0129 ± 0.0001 mg/mL), anti-HIV (IC 50  = 1.736 ± 0.036 µM), antitrypanosomal (IC 50  = 1.040 ± 0.010 µM) activities. Furthermore, SN did not present cytotoxic effect on HeLa cancer cell lines. The integrated strategy based on LC-ESI-QTOF-MS/MS provided a powerful tool and a multidimensional perspective for further exploration of active ingredients in S. nitidula responsible for the antioxidant, anti-HIV and antitrypanosomal activities.