Improvement of the antibody-dependent respiratory burst assay for assessing protective immune responses to malaria

The antibody-dependent respiratory burst (ADRB) assay is a sensitive isoluminol-based chemiluminescence (CL) functional assay designed to assess the capacity of opsonizing antibodies against merozoites to induce neutrophil respiratory burst. ADRB was shown to measure protective immunity against mala...

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Bibliographic Details
Published in:Open biology 2022-03, Vol.12 (3), p.210288-210288
Main Authors: Mansourou, Annick, Joos, Charlotte, Niass, Oumy, Diouf, Babacar, Tall, Adama, Perraut, Ronald, Niang, Makhtar, Toure-Balde, Aissatou
Format: Article
Language:English
Subjects:
Animals
Antibodies, Protozoan
functional assay
Humans
Immunity
malaria
Malaria - prevention & control
Merozoites
neutrophils
Plasmodium falciparum
Reproducibility of Results
Respiratory Burst
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Summary:The antibody-dependent respiratory burst (ADRB) assay is a sensitive isoluminol-based chemiluminescence (CL) functional assay designed to assess the capacity of opsonizing antibodies against merozoites to induce neutrophil respiratory burst. ADRB was shown to measure protective immunity against malaria in endemic areas, but the assay needed further improvement to ensure better sensitivity and reproducibility. Here, we adjusted parameters such as the freezing-thawing procedure of merozoites, merozoites's concentration and the buffer solution's pH, and we used the improved assay to measure ADRB activity of 207 sera from 97 and 110 individuals living, respectively, in Dielmo and Ndiop villages with differing malaria endemicity. The improvement led to increased CL intensity and assay sensitivity, and a higher reproducibility. In both areas, ADRB activity correlated with malaria endemicity and individual's age discriminated groups with and without clinical malaria episodes, and significantly correlated with clinical protection from malaria. Our results demonstrate that the improved ADRB assay can be valuably used to assess acquired immunity during monitoring by control programmes and/or clinical trials.
ISSN:2046-2441
2046-2441
DOI:10.1098/rsob.210288