Antibacterial and Anti-Inflammatory Properties of ZnO Nanoparticles Synthesized by a Green Method Using Sargassum Extracts

• Published in: International journal of molecular sciences 2023-01, Vol.24 (2), p.1474
• Main Authors: Lopez-Miranda, Jose Luis, Molina, Gustavo A, González-Reyna, Marlen Alexis, España-Sánchez, Beatriz Liliana, Esparza, Rodrigo, Silva, Rodolfo, Estévez, Miriam
• Format: Article
• Language: English
• Subjects: Algae; Anti-Bacterial Agents - chemistry; anti-inflammatory activity; Anti-inflammatory agents; Antibacterial activity; Diclofenac; green synthesis; Inflammation; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Morphology; Nanoparticles; Particle size; Plant Extracts - chemistry; Plant Extracts - pharmacology; Sargassum - chemistry; sargassum extracts; Scanning electron microscopy; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus; Ultrasonic imaging; Ultrasound; X-Ray Diffraction; Zinc oxide; Zinc Oxide - chemistry; Zinc Oxide - pharmacology; Zinc oxides; ZnO nanoparticles
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24021474

The present work shows the synthesis of ZnO nanoparticles through a green method, using extracts, which provide the reducing and stabilizing compounds. The conditions of the medium in which the reaction was carried out was evaluated, that is, magnetic stirring, ultrasound assisted, and resting condition. UV-Vis, FTIR spectroscopy, and X-ray diffraction results confirmed the synthesis of ZnO with nanometric crystal size. The scanning electron microscopy analysis showed that the morphology and size of the particles depends on the synthesis condition used. It obtained particles between 20 and 200 nm in the sample without agitation, while the samples with stirring and ultrasound were 80 nm and 100 nm, respectively. ZnO nanoparticles showed antibacterial activity against Gram-positive and Gram-negative . A quantitative analysis was performed by varying the concentration of ZnO nanoparticles. In all cases, the antibacterial activity against Gram-positives was greater than against Gram-negatives. Ultrasound-assisted ZnO nanoparticles showed the highest activity, around 99% and 80% for and , respectively. Similar results were obtained in the study of the anti-inflammatory activity of ZnO nanoparticles; the ultrasound-assisted sample exhibited the highest percentage (93%), even above that shown by diclofenac, which was used as a reference. Therefore, the ZnO nanoparticles synthesized with extracts have properties that can be used safely and efficiently in the field of biomedicine.