Calmodulin-Like (CML) Gene Family in Medicago truncatula : Genome-Wide Identification, Characterization and Expression Analysis

• Published in: International journal of molecular sciences 2020-10, Vol.21 (19), p.7142
• Main Authors: Sun, Qiguo, Yu, Shuhan, Guo, Zhenfei
• Format: Article
• Language: English
• Subjects: abiotic stress; Amino acids; Arabidopsis - genetics; Arabidopsis thaliana; Beans; Calcium ions; Calcium signalling; Calcium-binding protein; Calcium-Binding Proteins - chemistry; Calcium-Binding Proteins - genetics; Calmodulin; Calmodulin - chemistry; Calmodulin - genetics; Chromosomes; Chromosomes, Plant - genetics; CML; Drought; EF Hand Motifs; EF-hand; expression profiling; Flowers & plants; Gene Duplication; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genes, Plant; Genetic aspects; Genome-wide association studies; Genomes; Homology; Kinases; Legumes; Medicago truncatula; Medicago truncatula - genetics; Mimosaceae; Multigene Family; Observations; Oryza - genetics; Oryza sativa; Pathogens; Phylogenetics; Phylogeny; Plant Proteins - chemistry; Plant Proteins - genetics; Plant resistance; promoter cis-acting element; Proteins; Seeds; Signal transduction; Stress, Physiological - genetics; Structural analysis; Transcriptome
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21197142

Calcium is an important second messenger in mediating adaptation responses of plants to abiotic and biotic stresses. Calmodulin-like (CML) protein is an important calcium-signaling protein that can sense and decode Ca signal in plants. is a model legume plant; however, investigations of MtCML proteins are limited. Using genome analysis and BLAST database searches, fifty MtCML proteins that possess EF-hand motifs were identified. Phylogenetic analysis showed that CML homologs between , and shared close relationships. Gene structure analysis revealed that these genes contained one to four conserved EF-hand motifs. All are localized to eight chromosomes and underwent gene duplication. In addition, genes were differentially expressed in different tissues of . -acting elements in promoter region and expression analysis revealed the potential response of MtCML protein to abiotic stress and hormones. The results provide a basis of further functional research on the gene family and facilitate their potential use for applications in the genetic improvement on in drought, cold and salt stress environments.