COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT , TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells

RING-finger-type ubiquitin E3 ligase Constitutively Photomorphogenic 1 (COP1) and floral integrators such as FLOWERING LOCUS T (FT), TWIN SISTER OF FT (TSF) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) have been identified as regulators of stomatal movement. However, little is known about th...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2022-11, Vol.23 (23), p.15037
Main Authors: An, Yu-Yan, Li, Jing, Feng, Yu-Xin, Sun, Zhi-Mao, Li, Zhong-Qi, Wang, Xiao-Ting, Zhang, Mei-Xiang, He, Jun-Min
Format: Article
Language:English
Subjects:
Abscisic acid
Accumulation
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
COP1
darkness
Flowering
Gene Expression Regulation, Plant
guard cell signaling
Guard cells
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Hydrogen Peroxide - pharmacology
Mutants
Nitric oxide
Nitric Oxide - metabolism
Phosphatidylethanolamine Binding Protein - genetics
Plant Stomata - metabolism
Stomata
Transcription factors
Ubiquitin
Ubiquitin-protein ligase
Ultraviolet radiation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:RING-finger-type ubiquitin E3 ligase Constitutively Photomorphogenic 1 (COP1) and floral integrators such as FLOWERING LOCUS T (FT), TWIN SISTER OF FT (TSF) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) have been identified as regulators of stomatal movement. However, little is known about their roles and relationship in dark-induced stomatal closure. Here, we demonstrated that COP1 is required for dark-induced stomatal closure using mutant. The mutant closed stomata in response to exogenous nitric oxide (NO) but not hydrogen peroxide (H O ), and H O but not NO accumulated in in darkness, further indicating that COP1 acts downstream of H O and upstream of NO in dark-induced stomatal closure. Expression of , and in wild-type (WT) plants decreased significantly with dark duration time, but this process was blocked in . Furthermore, , , and mutants accumulated NO and closed stomata faster than WT plants in response to darkness. Altogether, our results indicate that COP1 transduces H O signaling, promotes NO accumulation in guard cells by suppressing , and expression, and consequently leads to stomatal closure in darkness. These findings add new insights into the mechanisms of dark-induced stomatal closure.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232315037