TmToll-7 Plays a Crucial Role in Innate Immune Responses Against Gram-Negative Bacteria by Regulating 5 AMP Genes in Tenebrio molitor

Although it is known that the Toll-7 receptor plays a critical role in antiviral autophagy, its function in other insects has not yet been reported. Here, we have identified a Toll-like receptor 7 gene, , in the coleopteran insect and examined its potential role in antibacterial and antifungal immun...

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Bibliographic Details
Published in:Frontiers in immunology 2019-03, Vol.10, p.310-310
Main Authors: Park, Soyi, Jo, Yong Hun, Park, Ki Beom, Ko, Hye Jin, Kim, Chang Eun, Bae, Young Min, Kim, Bobae, Jun, Sung Ah, Bang, In Seok, Lee, Yong Seok, Kim, Yu Jung, Han, Yeon Soo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
AMP assay
Animals
antimicrobial peptides
Base Sequence
Candida albicans - immunology
Escherichia coli - immunology
Gene Expression - immunology
Gram-Negative Bacteria - immunology
Immunity, Innate - genetics
Immunity, Innate - immunology
Immunology
Insect Proteins - immunology
Larva - immunology
Larva - microbiology
microbial infection
RNAi
Staphylococcus aureus - immunology
Tenebrio - immunology
Tenebrio - microbiology
Tenebrio molitor
Toll-7
Toll-Like Receptor 7 - genetics
Toll-Like Receptor 7 - immunology
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Summary:Although it is known that the Toll-7 receptor plays a critical role in antiviral autophagy, its function in other insects has not yet been reported. Here, we have identified a Toll-like receptor 7 gene, , in the coleopteran insect and examined its potential role in antibacterial and antifungal immunity. We showed that expression was significantly induced in larvae 6 h after infection with and and 9 h after infection with . However, even though was induced by all three pathogens, we found that knockdown significantly reduced larval survival to , but not to , and infections. To understand the reasons for this difference, we examined the effects of knockdown on antimicrobial peptide (AMP) gene expression and found a significant reduction of -induced expression of AMP genes such as , and . Furthermore, knockdown larvae infected with showed significantly higher bacterial growth in the hemolymph compared to control larvae treated with dsRNA. Taken together, our results suggest that TmToll-7 plays an important role in regulating the immune response of to .
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2019.00310