A Unique Gene-Silencing Approach, Using an Intelligent RNA Expression Device (iRed), Results in Minimal Immune Stimulation When Given by Local Intrapleural Injection in Malignant Pleural Mesothelioma

We have recently introduced an intelligent RNA expression device (iRed), comprising the minimum essential components needed to transcribe short hairpin RNA (shRNA) in cells. Use of iRed efficiently produced shRNA molecules after transfection into cells and alleviated the innate immune stimulation fo...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2020-04, Vol.25 (7), p.1725
Main Authors: Ando, Hidenori, Saito-Tarashima, Noriko, Lila, Amr S Abu, Kinjo, Nozomi, Shimizu, Taro, Ishima, Yu, Minakawa, Noriaki, Ishida, Tatsuhiro
Format: Article
Language:English
Subjects:
Animals
cationic liposomes
Cell Line, Tumor
Deoxyribonucleic acid
Disease Models, Animal
DNA
Gene Expression
Gene Silencing
Humans
Immunity, Innate - genetics
Immunomodulation - genetics
Injection
innate immunity
intelligent RNA expression device (iRed)
Interleukin 6
Intravenous administration
Liposomes
Mesothelioma
Mesothelioma, Malignant - genetics
Mice
Molecular weight
Plasmids
Pleural Neoplasms - genetics
Polyethylene glycol
Ratios
RNA Interference
RNA, Small Interfering - administration & dosage
RNA, Small Interfering - genetics
RNA-mediated interference
shRNA
Stimulation
Transfection
Xenograft Model Antitumor Assays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c603t-9af368a9b3645578c1edb82d9e4b96dde967d6fcbbbf22ab8b0c92f12ba412ef3
cites cdi_FETCH-LOGICAL-c603t-9af368a9b3645578c1edb82d9e4b96dde967d6fcbbbf22ab8b0c92f12ba412ef3
container_end_page
container_issue 7
container_start_page 1725
container_title Molecules (Basel, Switzerland)
container_volume 25
creator Ando, Hidenori
Saito-Tarashima, Noriko
Lila, Amr S Abu
Kinjo, Nozomi
Shimizu, Taro
Ishima, Yu
Minakawa, Noriaki
Ishida, Tatsuhiro
description We have recently introduced an intelligent RNA expression device (iRed), comprising the minimum essential components needed to transcribe short hairpin RNA (shRNA) in cells. Use of iRed efficiently produced shRNA molecules after transfection into cells and alleviated the innate immune stimulation following intravenous injection. To study the usefulness of iRed for local injection, the engineered iRed encoding luciferase shRNA (Luc iRed), complexed with cationic liposomes (Luc iRed/liposome-complexes), was intrapleurally injected into an orthotopic mesothelioma mouse model. Luc iRed/liposome-complexes markedly suppressed the expression of a luciferase marker gene in pleurally disseminated mesothelioma cells. The suppressive efficiency was correlated with the expression level of shRNA within the mesothelioma cells. In addition, intrapleural injection of iRed/liposome-complexes did not induce IL-6 production in the pleural space and consequently in the blood compartment, although plasmid DNA (pDNA) or dsDNA (the natural construct for iRed) in the formulation did. Local delivery of iRed could augment the in vivo gene silencing effect without eliciting pronounced innate immune stimulation. Our results might hold promise for widespread utilization of iRed as an RNAi-based therapeutic for intracelial malignant cancers.
doi_str_mv 10.3390/molecules25071725
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_ed08b878a69d480babcd551e30ddd4ce</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_ed08b878a69d480babcd551e30ddd4ce</doaj_id><sourcerecordid>2389466381</sourcerecordid><originalsourceid>FETCH-LOGICAL-c603t-9af368a9b3645578c1edb82d9e4b96dde967d6fcbbbf22ab8b0c92f12ba412ef3</originalsourceid><addsrcrecordid>eNplkttuEzEQhlcIRA_wANwgS9yA1IAPe_DeVIpKCZFSQCkRlysfZhNHXnuxd6P2CXktdpNQteLG9nj-_xuPPEnyhuCPjJX4U-MtqN5CpBkuSEGzZ8kpSSmeMJyWzx-dT5KzGLcYU5KS7GVywijlrMDlafJnilbO_O4BzcDB5NZYcMq4NZq2bfBCbS7QKo6xcGjuOrDWrMF1aPltiq7v2gAxGu_QZ9gZBei9WYL-cIGWEHvbRWQcujHONMKiedP0DtBtZ5reim40_dqAQzOzG1Z5jxZejTLXBdFa6MM-2ILaS0eQGEo7MdT-cUzfQPTdBqzxjXiVvKiFjfD6uJ8nqy_XP6--ThbfZ_Or6WKicsy6SSlqlnNRSpanWVZwRUBLTnUJqSxzraHMC53XSkpZUyokl1iVtCZUipRQqNl5Mj9wtRfbqg1Db-G-8sJU-wsf1pUInVEWKtCYS15wkZc65VgKqXSWEWBYa50qGFiXB1bbywa0grF3-wT6NOPMplr7XVUQTmiKB8C7IyD44QtjV219H9zQf0UZL9M8Z5wMKnJQqeBjDFA_VCC4Gueo-m-OBs_bx097cPwbHPYXTovLdg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2389466381</pqid></control><display><type>article</type><title>A Unique Gene-Silencing Approach, Using an Intelligent RNA Expression Device (iRed), Results in Minimal Immune Stimulation When Given by Local Intrapleural Injection in Malignant Pleural Mesothelioma</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>PubMed Central</source><creator>Ando, Hidenori ; Saito-Tarashima, Noriko ; Lila, Amr S Abu ; Kinjo, Nozomi ; Shimizu, Taro ; Ishima, Yu ; Minakawa, Noriaki ; Ishida, Tatsuhiro</creator><creatorcontrib>Ando, Hidenori ; Saito-Tarashima, Noriko ; Lila, Amr S Abu ; Kinjo, Nozomi ; Shimizu, Taro ; Ishima, Yu ; Minakawa, Noriaki ; Ishida, Tatsuhiro</creatorcontrib><description>We have recently introduced an intelligent RNA expression device (iRed), comprising the minimum essential components needed to transcribe short hairpin RNA (shRNA) in cells. Use of iRed efficiently produced shRNA molecules after transfection into cells and alleviated the innate immune stimulation following intravenous injection. To study the usefulness of iRed for local injection, the engineered iRed encoding luciferase shRNA (Luc iRed), complexed with cationic liposomes (Luc iRed/liposome-complexes), was intrapleurally injected into an orthotopic mesothelioma mouse model. Luc iRed/liposome-complexes markedly suppressed the expression of a luciferase marker gene in pleurally disseminated mesothelioma cells. The suppressive efficiency was correlated with the expression level of shRNA within the mesothelioma cells. In addition, intrapleural injection of iRed/liposome-complexes did not induce IL-6 production in the pleural space and consequently in the blood compartment, although plasmid DNA (pDNA) or dsDNA (the natural construct for iRed) in the formulation did. Local delivery of iRed could augment the in vivo gene silencing effect without eliciting pronounced innate immune stimulation. Our results might hold promise for widespread utilization of iRed as an RNAi-based therapeutic for intracelial malignant cancers.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules25071725</identifier><identifier>PMID: 32283709</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; cationic liposomes ; Cell Line, Tumor ; Deoxyribonucleic acid ; Disease Models, Animal ; DNA ; Gene Expression ; Gene Silencing ; Humans ; Immunity, Innate - genetics ; Immunomodulation - genetics ; Injection ; innate immunity ; intelligent RNA expression device (iRed) ; Interleukin 6 ; Intravenous administration ; Liposomes ; Mesothelioma ; Mesothelioma, Malignant - genetics ; Mice ; Molecular weight ; Plasmids ; Pleural Neoplasms - genetics ; Polyethylene glycol ; Ratios ; RNA Interference ; RNA, Small Interfering - administration &amp; dosage ; RNA, Small Interfering - genetics ; RNA-mediated interference ; shRNA ; Stimulation ; Transfection ; Xenograft Model Antitumor Assays</subject><ispartof>Molecules (Basel, Switzerland), 2020-04, Vol.25 (7), p.1725</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c603t-9af368a9b3645578c1edb82d9e4b96dde967d6fcbbbf22ab8b0c92f12ba412ef3</citedby><cites>FETCH-LOGICAL-c603t-9af368a9b3645578c1edb82d9e4b96dde967d6fcbbbf22ab8b0c92f12ba412ef3</cites><orcidid>0000-0002-5359-3722 ; 0000-0001-7385-868X ; 0000-0002-8326-2166</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2389466381/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2389466381?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25732,27903,27904,36991,44569,53769,53771,74872</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32283709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ando, Hidenori</creatorcontrib><creatorcontrib>Saito-Tarashima, Noriko</creatorcontrib><creatorcontrib>Lila, Amr S Abu</creatorcontrib><creatorcontrib>Kinjo, Nozomi</creatorcontrib><creatorcontrib>Shimizu, Taro</creatorcontrib><creatorcontrib>Ishima, Yu</creatorcontrib><creatorcontrib>Minakawa, Noriaki</creatorcontrib><creatorcontrib>Ishida, Tatsuhiro</creatorcontrib><title>A Unique Gene-Silencing Approach, Using an Intelligent RNA Expression Device (iRed), Results in Minimal Immune Stimulation When Given by Local Intrapleural Injection in Malignant Pleural Mesothelioma</title><title>Molecules (Basel, Switzerland)</title><addtitle>Molecules</addtitle><description>We have recently introduced an intelligent RNA expression device (iRed), comprising the minimum essential components needed to transcribe short hairpin RNA (shRNA) in cells. Use of iRed efficiently produced shRNA molecules after transfection into cells and alleviated the innate immune stimulation following intravenous injection. To study the usefulness of iRed for local injection, the engineered iRed encoding luciferase shRNA (Luc iRed), complexed with cationic liposomes (Luc iRed/liposome-complexes), was intrapleurally injected into an orthotopic mesothelioma mouse model. Luc iRed/liposome-complexes markedly suppressed the expression of a luciferase marker gene in pleurally disseminated mesothelioma cells. The suppressive efficiency was correlated with the expression level of shRNA within the mesothelioma cells. In addition, intrapleural injection of iRed/liposome-complexes did not induce IL-6 production in the pleural space and consequently in the blood compartment, although plasmid DNA (pDNA) or dsDNA (the natural construct for iRed) in the formulation did. Local delivery of iRed could augment the in vivo gene silencing effect without eliciting pronounced innate immune stimulation. Our results might hold promise for widespread utilization of iRed as an RNAi-based therapeutic for intracelial malignant cancers.</description><subject>Animals</subject><subject>cationic liposomes</subject><subject>Cell Line, Tumor</subject><subject>Deoxyribonucleic acid</subject><subject>Disease Models, Animal</subject><subject>DNA</subject><subject>Gene Expression</subject><subject>Gene Silencing</subject><subject>Humans</subject><subject>Immunity, Innate - genetics</subject><subject>Immunomodulation - genetics</subject><subject>Injection</subject><subject>innate immunity</subject><subject>intelligent RNA expression device (iRed)</subject><subject>Interleukin 6</subject><subject>Intravenous administration</subject><subject>Liposomes</subject><subject>Mesothelioma</subject><subject>Mesothelioma, Malignant - genetics</subject><subject>Mice</subject><subject>Molecular weight</subject><subject>Plasmids</subject><subject>Pleural Neoplasms - genetics</subject><subject>Polyethylene glycol</subject><subject>Ratios</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering - administration &amp; dosage</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA-mediated interference</subject><subject>shRNA</subject><subject>Stimulation</subject><subject>Transfection</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNplkttuEzEQhlcIRA_wANwgS9yA1IAPe_DeVIpKCZFSQCkRlysfZhNHXnuxd6P2CXktdpNQteLG9nj-_xuPPEnyhuCPjJX4U-MtqN5CpBkuSEGzZ8kpSSmeMJyWzx-dT5KzGLcYU5KS7GVywijlrMDlafJnilbO_O4BzcDB5NZYcMq4NZq2bfBCbS7QKo6xcGjuOrDWrMF1aPltiq7v2gAxGu_QZ9gZBei9WYL-cIGWEHvbRWQcujHONMKiedP0DtBtZ5reim40_dqAQzOzG1Z5jxZejTLXBdFa6MM-2ILaS0eQGEo7MdT-cUzfQPTdBqzxjXiVvKiFjfD6uJ8nqy_XP6--ThbfZ_Or6WKicsy6SSlqlnNRSpanWVZwRUBLTnUJqSxzraHMC53XSkpZUyokl1iVtCZUipRQqNl5Mj9wtRfbqg1Db-G-8sJU-wsf1pUInVEWKtCYS15wkZc65VgKqXSWEWBYa50qGFiXB1bbywa0grF3-wT6NOPMplr7XVUQTmiKB8C7IyD44QtjV219H9zQf0UZL9M8Z5wMKnJQqeBjDFA_VCC4Gueo-m-OBs_bx097cPwbHPYXTovLdg</recordid><startdate>20200409</startdate><enddate>20200409</enddate><creator>Ando, Hidenori</creator><creator>Saito-Tarashima, Noriko</creator><creator>Lila, Amr S Abu</creator><creator>Kinjo, Nozomi</creator><creator>Shimizu, Taro</creator><creator>Ishima, Yu</creator><creator>Minakawa, Noriaki</creator><creator>Ishida, Tatsuhiro</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5359-3722</orcidid><orcidid>https://orcid.org/0000-0001-7385-868X</orcidid><orcidid>https://orcid.org/0000-0002-8326-2166</orcidid></search><sort><creationdate>20200409</creationdate><title>A Unique Gene-Silencing Approach, Using an Intelligent RNA Expression Device (iRed), Results in Minimal Immune Stimulation When Given by Local Intrapleural Injection in Malignant Pleural Mesothelioma</title><author>Ando, Hidenori ; Saito-Tarashima, Noriko ; Lila, Amr S Abu ; Kinjo, Nozomi ; Shimizu, Taro ; Ishima, Yu ; Minakawa, Noriaki ; Ishida, Tatsuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c603t-9af368a9b3645578c1edb82d9e4b96dde967d6fcbbbf22ab8b0c92f12ba412ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>cationic liposomes</topic><topic>Cell Line, Tumor</topic><topic>Deoxyribonucleic acid</topic><topic>Disease Models, Animal</topic><topic>DNA</topic><topic>Gene Expression</topic><topic>Gene Silencing</topic><topic>Humans</topic><topic>Immunity, Innate - genetics</topic><topic>Immunomodulation - genetics</topic><topic>Injection</topic><topic>innate immunity</topic><topic>intelligent RNA expression device (iRed)</topic><topic>Interleukin 6</topic><topic>Intravenous administration</topic><topic>Liposomes</topic><topic>Mesothelioma</topic><topic>Mesothelioma, Malignant - genetics</topic><topic>Mice</topic><topic>Molecular weight</topic><topic>Plasmids</topic><topic>Pleural Neoplasms - genetics</topic><topic>Polyethylene glycol</topic><topic>Ratios</topic><topic>RNA Interference</topic><topic>RNA, Small Interfering - administration &amp; dosage</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA-mediated interference</topic><topic>shRNA</topic><topic>Stimulation</topic><topic>Transfection</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ando, Hidenori</creatorcontrib><creatorcontrib>Saito-Tarashima, Noriko</creatorcontrib><creatorcontrib>Lila, Amr S Abu</creatorcontrib><creatorcontrib>Kinjo, Nozomi</creatorcontrib><creatorcontrib>Shimizu, Taro</creatorcontrib><creatorcontrib>Ishima, Yu</creatorcontrib><creatorcontrib>Minakawa, Noriaki</creatorcontrib><creatorcontrib>Ishida, Tatsuhiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Molecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ando, Hidenori</au><au>Saito-Tarashima, Noriko</au><au>Lila, Amr S Abu</au><au>Kinjo, Nozomi</au><au>Shimizu, Taro</au><au>Ishima, Yu</au><au>Minakawa, Noriaki</au><au>Ishida, Tatsuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Unique Gene-Silencing Approach, Using an Intelligent RNA Expression Device (iRed), Results in Minimal Immune Stimulation When Given by Local Intrapleural Injection in Malignant Pleural Mesothelioma</atitle><jtitle>Molecules (Basel, Switzerland)</jtitle><addtitle>Molecules</addtitle><date>2020-04-09</date><risdate>2020</risdate><volume>25</volume><issue>7</issue><spage>1725</spage><pages>1725-</pages><issn>1420-3049</issn><eissn>1420-3049</eissn><abstract>We have recently introduced an intelligent RNA expression device (iRed), comprising the minimum essential components needed to transcribe short hairpin RNA (shRNA) in cells. Use of iRed efficiently produced shRNA molecules after transfection into cells and alleviated the innate immune stimulation following intravenous injection. To study the usefulness of iRed for local injection, the engineered iRed encoding luciferase shRNA (Luc iRed), complexed with cationic liposomes (Luc iRed/liposome-complexes), was intrapleurally injected into an orthotopic mesothelioma mouse model. Luc iRed/liposome-complexes markedly suppressed the expression of a luciferase marker gene in pleurally disseminated mesothelioma cells. The suppressive efficiency was correlated with the expression level of shRNA within the mesothelioma cells. In addition, intrapleural injection of iRed/liposome-complexes did not induce IL-6 production in the pleural space and consequently in the blood compartment, although plasmid DNA (pDNA) or dsDNA (the natural construct for iRed) in the formulation did. Local delivery of iRed could augment the in vivo gene silencing effect without eliciting pronounced innate immune stimulation. Our results might hold promise for widespread utilization of iRed as an RNAi-based therapeutic for intracelial malignant cancers.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32283709</pmid><doi>10.3390/molecules25071725</doi><orcidid>https://orcid.org/0000-0002-5359-3722</orcidid><orcidid>https://orcid.org/0000-0001-7385-868X</orcidid><orcidid>https://orcid.org/0000-0002-8326-2166</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1420-3049
ispartof Molecules (Basel, Switzerland), 2020-04, Vol.25 (7), p.1725
issn 1420-3049
1420-3049
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_ed08b878a69d480babcd551e30ddd4ce
source Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central
subjects Animals
cationic liposomes
Cell Line, Tumor
Deoxyribonucleic acid
Disease Models, Animal
DNA
Gene Expression
Gene Silencing
Humans
Immunity, Innate - genetics
Immunomodulation - genetics
Injection
innate immunity
intelligent RNA expression device (iRed)
Interleukin 6
Intravenous administration
Liposomes
Mesothelioma
Mesothelioma, Malignant - genetics
Mice
Molecular weight
Plasmids
Pleural Neoplasms - genetics
Polyethylene glycol
Ratios
RNA Interference
RNA, Small Interfering - administration & dosage
RNA, Small Interfering - genetics
RNA-mediated interference
shRNA
Stimulation
Transfection
Xenograft Model Antitumor Assays
title A Unique Gene-Silencing Approach, Using an Intelligent RNA Expression Device (iRed), Results in Minimal Immune Stimulation When Given by Local Intrapleural Injection in Malignant Pleural Mesothelioma
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T11%3A31%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Unique%20Gene-Silencing%20Approach,%20Using%20an%20Intelligent%20RNA%20Expression%20Device%20(iRed),%20Results%20in%20Minimal%20Immune%20Stimulation%20When%20Given%20by%20Local%20Intrapleural%20Injection%20in%20Malignant%20Pleural%20Mesothelioma&rft.jtitle=Molecules%20(Basel,%20Switzerland)&rft.au=Ando,%20Hidenori&rft.date=2020-04-09&rft.volume=25&rft.issue=7&rft.spage=1725&rft.pages=1725-&rft.issn=1420-3049&rft.eissn=1420-3049&rft_id=info:doi/10.3390/molecules25071725&rft_dat=%3Cproquest_doaj_%3E2389466381%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c603t-9af368a9b3645578c1edb82d9e4b96dde967d6fcbbbf22ab8b0c92f12ba412ef3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2389466381&rft_id=info:pmid/32283709&rfr_iscdi=true