Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population

A novel pestivirus species, termed Lateral-shaking Inducing Neuro-Degenerative Agent virus (LindaV), was discovered in a piglet-producing farm in Austria in 2015 related to severe congenital tremor cases. Since the initial outbreak LindaV has not been found anywhere else. In this study, we determine...

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Bibliographic Details
Published in:Viruses 2021-05, Vol.13 (6), p.1001
Main Authors: Kiesler, Alexandra, Plankensteiner, Jakob, Schwarz, Lukas, Riedel, Christiane, Seitz, Kerstin, Mötz, Marlene, Ladinig, Andrea, Lamp, Benjamin, Rümenapf, Till
Format: Article
Language:English
Subjects:
Antibodies
Cell culture
Cloning
Congenital diseases
Genomes
Hogs
Immunocompetence
Infections
Linda virus
novel Linda virus strain
pestiviruses
Plasmids
Population
Serology
seroprevalence
serum virus neutralization assay
Tremor
Viruses
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Summary:A novel pestivirus species, termed Lateral-shaking Inducing Neuro-Degenerative Agent virus (LindaV), was discovered in a piglet-producing farm in Austria in 2015 related to severe congenital tremor cases. Since the initial outbreak LindaV has not been found anywhere else. In this study, we determined the seroprevalence of LindaV infections in the domestic pig population of Austria. A fluorophore labeled infectious cDNA clone of LindaV (mCherry-LindaV) was generated and used in serum virus neutralization (SVN) assays for the detection of LindaV specific neutralizing antibodies in porcine serum samples. In total, 637 sera from sows and gilts from five federal states of Austria, collected between the years 2015 and 2020, were analyzed. We identified a single serum showing a high neutralizing antibody titer, that originated from a farm (Farm S2) in the proximity of the initially affected farm. The analysis of 57 additional sera from Farm S2 revealed a wider spread of LindaV in this pig herd. Furthermore, a second LindaV strain originating from this farm could be isolated in cell culture and was further characterized at the genetic level. Possible transmission routes and virus reservoir hosts of this emerging porcine virus need to be addressed in future studies.
ISSN:1999-4915
1999-4915
DOI:10.3390/v13061001