Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines

The aim of this study was to assess the biological effect of organically coated Grias neuberthii (piton) fruit and Persea americana (avocado) leaves nanoparticles (NPs) on cervical cancer (HeLa) and breast adenocarcinoma (MCF-7) cells with an emphasis on gene expression (p53 transcription factor and...

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Bibliographic Details
Published in:Journal of nanotechnology 2018-01, Vol.2018 (2018), p.1-11
Main Authors: Castillo, Luis, Grijalva, Marcelo, Vallejo, María José, Salazar, Lizeth, Maldonado, Alexander
Format: Article
Language:English
Subjects:
Antioxidants
Apoptosis
Biological effects
Biosynthesis
Biotechnology
Cancer
Cancer therapies
Cell cycle
Coating effects
Cytotoxicity
Drug dosages
Gene expression
Glutathione
Herbal medicine
Medical research
Nanoparticles
Oxidative stress
Silver
Toxicity
Toxicology
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Summary:The aim of this study was to assess the biological effect of organically coated Grias neuberthii (piton) fruit and Persea americana (avocado) leaves nanoparticles (NPs) on cervical cancer (HeLa) and breast adenocarcinoma (MCF-7) cells with an emphasis on gene expression (p53 transcription factor and glutathione-S-transferase GST) and cell viability. UV-Vis spectroscopy analysis showed that synthesized AgNPs remained partially stable under cell culture conditions. HeLa cells remained viable when exposed to piton and avocado AgNPs. A statistically significant, dose-dependent cytotoxic response to both AgNPs was found on the breast cancer (MCF-7) cell line at concentrations above 50 µM. While expression levels of transcription factor p53 showed downregulation in treated MCF-7 and HeLa cells, GST expression was not affected in both cell lines treated. Cell viability assays along with gene expression levels in treated MCF-7 cells support a cancer cell population undergoing cell cycle arrest. The selective toxicity of biosynthesized piton/avocado AgNPs on MCF-7 cells might be of value for novel therapeutics.
ISSN:1687-9503
1687-9511
DOI:10.1155/2018/9689131